七叶皂苷对大鼠肝微粒体细胞色素 P450酶含量及活性的影响  被引量:3

Effects of Aescine on the content and activity of cytochrome P450 in livermicrosome of rats

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作  者:吴秀君[1,2] 肇丽梅[2] 崔相勇[3] 高峰[3] 顾景凯[3] 

机构地区:[1]辽宁中医药大学附属医院科技管理部,沈阳110032 [2]中国医科大学附属盛京医院药学部,沈阳110042 [3]吉林大学生命科学学院,长春130061

出  处:《中国临床药理学杂志》2014年第7期620-622,631,共4页The Chinese Journal of Clinical Pharmacology

基  金:国家自然科学基金资助项目(30772613)

摘  要:目的考察七叶皂苷对大鼠肝微粒体的蛋白含量、CYP450酶总量和主要CYP450酶亚型(CYP1A2、CYP2D6、CYP3A4和CYP2C19)活性的影响。方法分别以0.9%NaCl和苯巴比妥作为空白组和阳性对照组,并以乙醇-5%葡萄糖溶液作为溶剂对照组,大鼠灌胃给予七叶皂苷(4.0 mg·kg-1·d-1),连续7 d,测定各组肝微粒体蛋白含量、CYP450蛋白含量以及CYP1A2、CYP2D6、CYP3A4及CYP2C19活性,并进行比较。结果与溶剂对照组相比,七叶皂苷给药组大鼠肝微粒体蛋白含量略增加,但差异无统计学意义(P>0.05);肝微粒体CYP450含量无明显差异。七叶皂苷给药后,CYP1A2和CYP3A4活性增加,但无统计学意义(P>0.05);CYP2D6和CYP2C19的活性没有改变。结论七叶皂苷对大鼠肝微粒体蛋白浓度、CYP450酶总量和CYP450酶亚型的活性均无影响。Objective To study the effect of Aescine on the content of livermicrosome protein , cytochrome P450 ( CYP450 ) and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome of rat liver.Methods Rats were given Aescine by oral administration ( 4.0 mg· kg-1 · d-1 ) for 7 days to detect the content of livermicrosome pro-tein and CYP450 and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in cytomicrosome taking 0.9%NaCl, ethanol-5%glucose so-lution and phenobarbital as blank , solvent and positive control group , re-spectively.Results Compared to solvent control group , Aescine in-creased the content of livermicrosome protein , however , it had no statisti-cal significance.Aescine had no effect on the content of CYP 450 , and the activity of CYP1A2, CYP2D6, CYP3A4 and CYP2C19 in solvent control group and Aescine group was comparable.Conclusions Aescine showed no effect on CYP 450 in rats.

关 键 词:七叶皂苷 肝微粒体 细胞色素P450 三萜类皂苷 

分 类 号:R285.577.9[医药卫生—中药学]

 

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