链霉菌H8797产生的二酮哌嗪类化合物的分离纯化和结构鉴定  

Isolation and structure identification of diketoperazine derivative produced by Streptomyces sp. H8797

作　　者：翟龙飞[1] 刘瑜[1] 张新宜[1] 詹良静[1] 田敏[1] 

机构地区：[1]四川抗菌素工业研究所,成都610052

出　　处：《中国抗生素杂志》2014年第7期486-489,共4页Chinese Journal of Antibiotics

基　　金：四川省国际科技合作计划项目(No.2011HH0013)资助

摘　　要：目的研究链霉菌H8797发酵液中抑制LFA-1/ICAM-1介导的人B淋巴瘤JY细胞聚集的活性成分。方法利用HP-20大孔吸附树脂吸附发酵液中的活性成分,梯度解吸,分部收集,跟踪监测活性,再通过C18硅胶柱层析和LH-20柱层析分离,高效制备液相色谱精制,测定活性,通过紫外、红外、元素分析、高分辨质谱、核磁共振进行分析,得到活性单组份。结果分离纯化了活性化合物H8797A并对其进行了结构确证。结论化合物H8797A为首次从天然产物中分离获得,属于二酮哌嗪类化合物,具有抑制LFA-1/ICAM-1介导的人B淋巴瘤JY细胞聚集的活性。Objective To study the active compound which inhibits the LFA-1/ICAM-1 mediated human B lymphoma JY cell aggregation from fermentation broth of Streptomyces sp. H8797. Methods Active components were absorbed by macroporous absorption resin HP-20 and gradient eluted, fraction collection, tracking and monitoring activity. Purified component were isolated by LH-20 column chromatography and preparative HPLC. Active compound was elucidated by elemental analysis and spectral analysis. Results The active compound H8797A was isolated and identificated. Conclusion H8797A was identified as a diketoperazine derivative and isolated from natural products for the first time. It inhibits LFA-1/ICAM-1 mediated human B lymphoma JY cell aggregation.

关 键 词：淋巴细胞功能相关因子-1 细胞间粘附分子-1 放线菌H8797 H8797A 二酮哌嗪 

分 类 号：R978.7[医药卫生—药品]