链霉菌HCCB10043中调控基因dptR1、dptR2及dptR3对A21978C合成的影响  

Influence of regulatory genes dptR1, dptR2 and dptR3 on A21978C production in Streptomyces sp. HCCB10043

作　　者：靳旭[1,2] 魏维[2,3] 饶敏[2,3] 陈玲玲[2,3] 戈梅[2,3] 

机构地区：[1]南京农业大学生命科学学院,南京210095 [2]上海来益生物药物研究开发中心,上海201203 [3]上海交通大学药学院,上海200240

出　　处：《中国抗生素杂志》2014年第7期490-493,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(81102354)

摘　　要：目的研究A21978C生物合成基因簇内3个调控基因dptR1、dptR2及dptR3对A1978C合成的影响。方法利用同源重组方法敲除这3个调控基因,用HPLC检测突变株A21978C产量的变化。结果 dptR1和dptR2敲除后突变株A21978C产量与野生株HCCB10043相比变化不大,dptR3敲除后突变株不产A21978C。结论 dptR3对达托霉素的合成至关重要,是一个正调控基因。Objective To investigate the influence ofA21978C biosynthesis gene clusters of 3 regulatory genes dptR1, dptR2 and dptR3 on synthesis ofA1978C. Methods dptR1, dptR2 and dptR3 were deleted by homologous recombination and the production ofA21978C in the corresponding mutants was detected via HPLC. Results There were three AdptR1 mutants, four AdptR2 mutants and two AdptR3 mutants obtained. The production of A21978C in AdptR1 and AdptR2 mutants was at a similar level to that of the wild-type strain, while the AdptR3 mutant lost its ability to produce A21978C. Conclusion dptR3 was essential forA21978C production and was a positive regulatory gene.

关 键 词：A21978C 调控基因 基因敲除 

分 类 号：Q93[生物学—微生物学]