活血生肌中药外用对大鼠失神经支配创面角蛋白19表达的影响  

Effect of the External Use of Chinese Traditional Medicine on Keratin 19 of the Denervated Wound of Rats

作　　者：董黎强[1] 成信法[2] 尹航[1] 王昌兴[1] 胡伟锋[1] 

机构地区：[1]浙江中医药大学附属第二医院骨一科,杭州310053 [2]浙江中医药大学

出　　处：《浙江中西医结合杂志》2014年第7期573-575,582,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省中医药科技计划项目(No.2009CA035)

摘　　要：目的观察活血生肌外用中药对大鼠失神经支配创面角蛋白19(k19)阳性表达的影响。方法 168只SD大鼠随机分成龙血膏组、生肌膏组、失神经组和正常组,每组42只。对前三组大鼠制造背部失神经支配的深创面模型,之后各组创面分别以龙血生肌膏、生肌愈皮膏、凡士林外用治疗;正常组大鼠背部制造深创面模型,以凡士林治疗。观察不同时间点各组创面愈合率、创面组织k19阳性表达率。结果各时间点正常组两中药组创面愈合率均高于失神经组(P<0.05,P<0.01)。龙血膏组、生肌膏组k19阳性表达于第11天达峰值,失神经组和正常组第15天达峰值。第4、7、11、15天时龙血膏组、生肌膏组k19阳性表达率明显高于失神经组和正常组(P<0.05,P<0.01),失神经组低于正常组(P<0.05,P<0.01)。结论活血生肌外用中药对失神经支配创面的愈合有促进作用,可能与外用中药增强了失神经支配创面的表皮干细胞增殖与分化有关。Objective To investigate the effect of the external use of Chinese medicine on the expression of Keratin 19（K19） in denervated wound healing of rats. Methods One hundred and sixty-eight SD rats were randomly divided into 4 groups： A group（longxuegao group）,B group（shengjigao group）,C group（denervated group）, and D group（normal group）,42 rats in each group. A wound with denervated whole layer skin defection was established on the back of every rat in A,B,and C groups,while a wound with whole layer skin defection was established on the back of every rat in D group. The rat in A and B groups were externally treated with the corresponding drugs,and the rat in C and D groups were treated with vaseline. The healing rates of wound were calcu-lated and the level of K19 expression was determined by immunohistochemistry at different time points after intervention. Results The healing rate of A,B,and D groups were higher than that of C group（P〈0.05）. The level of K19 expression of A and B groups reached the peak on d11,which was earlier than that of C and D groups（on d15）.On d4,d7,d11,d15,the level of K19 expression of A and B groups was significantly higher than that of C and D groups（P〈0.05 or P〈0.01）,and that of C group was lower than that of D group（P〈0.05 or P〈0.01）. Conclusion The external use of Chinese medicine can promote wound healing of denervation. Its mechanism may be related to enhancing proliferation and differentiation of epidermal stem cells in the healing process of the wound of denervation.

关 键 词：大鼠 创面 去神经支配 生肌愈皮膏 龙血生肌膏 角蛋白19 

分 类 号：R285.5[医药卫生—中药学]