柿叶黄酮对同型半胱氨酸诱导小胶质细胞细胞因子表达的影响  

Flavone from Diospyros kaki Leaves Represses the Expression of Cytokines Induced by Homocysteine in Mi-croglia

作　　者：李颖[1] 毛瑞阳[2] 杜晓红[2] 赵晓薇[2] 

机构地区：[1]浙江医院干部科,杭州310013 [2]温州医科大学附属第一医院

出　　处：《浙江中西医结合杂志》2014年第7期576-579,共4页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：浙江省温州市科技计划项目(No.Y20100056)

摘　　要：目的观察柿叶黄酮对同型半胱氨酸诱导的小胶质细胞表达白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的影响,并与银杏叶提取物EGb761进行比较。方法将体外培养的小鼠小胶质细胞(BV-2细胞)分成空白对照组、同型半胱氨酸组(Hcy)、Hcy+EGb761组(100mg/L)和Hcy+低、中、高(12.5、25、50μg/mL)剂量柿叶黄酮组,培养72h。应用RT-qPCR方法评价各组IL-1β、IL-6和TNF-α的mRNA表达,酶联免疫吸附法(ELISA)测定培养上清液中上述细胞因子的蛋白浓度。结果与空白对照组比较,同型半胱氨酸组细胞内IL-1β、IL-6和TNF-α的mRNA表达和细胞培养上清液中蛋白浓度明显增加(P<0.05);与同型半胱氨酸组比较,Hcy+EGb761组和Hcy+低、中、高剂量柿叶黄酮组各细胞因子mRNA表达及培养上清液中蛋白浓度均降低(P<0.05),尤其是高剂量柿叶黄酮组结果与Hcy+EGb761组作用相似。结论柿叶黄酮能抑制同型半胱氨酸诱导小胶质细胞表达IL-1β、IL-6和TNF-α,在一定浓度条件下其作用不亚于银杏叶提取物。Objective To evaluate the effect of flavone from diospyros kaki leaves on the expression of interleukin-1β（IL-1β）,interleukin-6（IL-6） and tumor necrosis factor-α（TNF-α） in microgia（BV-2 cells） induced by homocysteine,and compared its effect with that of EGb761. Methods The BV-2 cells incubated in vitro were di vided into six groups：blank control group,homocysteine（Hcy） group,EGb761 group（100mg/L） and flavone from diospyros kaki groups of different concentration（12.5,25,50μg/mL）. After 72h incubation,the mRNA expression of IL-1β,IL-6 and TNF-α was assessed by RT-qPCR and the protein of cytokines in supernatant was detected by ELISA. Results The mRNA expression and supernatant protein of IL-1β,IL-6,TNF-α in Hcy group were significantly higher than those of control group（P〈0.05）,but were significantly decreased in EGb761 group and flavone from diospyros kaki groups compared with those of Hcy group（P〈0.05）. Especially,both the mRNA expression and supernatant protein in higher concentration flavone from diospyros kaki leaf group were comparable to those in EGb761 group. Conclusion Flavone from diospyros kaki leaves can inhibit the expression of IL-1β,IL-6 and TNF-α in cultured BV-2 cells induced by Hcy. In a certain concentration,it was no less effective than EGb761.

关 键 词：小胶质细胞 柿叶黄酮 同型半胱氨酸 白介素-1Β 白介素-6 肿瘤坏死因子-α 

分 类 号：R285[医药卫生—中药学]