粉纹夜蛾中肠微生物中产AHLs群体感应信号分子菌株的分离鉴定  

Isolation and Identification of Accumulation of Quorum Signal Molecules Strains from the Trichoplusia ni Midgut

作　　者：黄媛媛[1,2] 马宏[1,2] 贾振华[1,2] 黄亚丽[1,2] 宋水山[1,2] 张霞[1,2] 

机构地区：[1]河北省科学院生物研究所,石家庄050081 [2]河北省主要农作物病害微生物控制工程技术研究中心,石家庄050081

出　　处：《生物技术通报》2014年第7期196-200,共5页Biotechnology Bulletin

基　　金：河北省自然科学基金项目(C2011302008)

摘　　要：利用紫色杆菌(Chromobacterium violaceum CV026)报告系统从粉纹夜蛾(Trichoplusia ni)中肠中分离出两株可以产群体感应信号分子的菌株Tni-9和Se-9。通过16S rDNA序列分析结果可知,分离菌株均与蒙氏肠球菌(Enterococcus mundtiiATCC 43186)有高度同源性,在系统发育树内位于同一分支。选取菌株Se-9进行生理生化特性鉴定,进一步证实该菌株为蒙氏肠球菌。将菌株Se-9进行薄层层析分析,发现该菌株可以产生两种群体感应信号分子C6-HSL和3OC6-HSL。Two strains named Tni-9 and Se-9 were isolated from Trichoplusia ni midgut by CV026 report system. The 16S rDNA sequences of the isolates were studied, blast analysis and the phylogenetic tree showed their 16S rDNA were highly homologous to that of Enterococcus mundtii ATCC 43186. By the physiological and biochemical characteristics, the strain Se-9 was further confirmed belong to Enterococcus mundtii .TLC analysis present that Se-9 could produce C6-HSL and 3OC6-HSL quorum sensing signals respecctively.

关 键 词：粉纹夜蛾 中肠微生物 群体感应 薄层层析 分离 鉴定 

分 类 号：S482.39[农业科学—农药学]