炎症性肠病患者髓系细胞表达触发受体-1与COX-2的表达及其意义  

The expression and significance of triggering receptor-1 and COX-2 in myeloid cells in patients with inflammatory bowel disease

作　　者：邢亚威[1] 刘春玉[1] 张月花[1] 

机构地区：[1]河北医科大学第四医院,河北石家庄050011

出　　处：《现代预防医学》2014年第14期2598-2600,共3页Modern Preventive Medicine

基　　金：河北省卫生厅科研项目(20090516)

摘　　要：目的研究炎症性肠病(Inflammatory Bowel Disease,IBD)患者肠黏膜髓系细胞表达触发受体-1(TREM-1)与环氧化酶-2(COX-2)的表达,以及两者与溃疡性结肠炎患者病情严重程度和内镜分级程度的关系。方法分别采用流式细胞技术和免疫组化方法对60例IBD患者和30例对照组患者肠黏膜中TREM-1和COX-2的表达进行测定,其中60例IBD患者中,包括52例溃疡性结肠炎(Ulcerative Colitis,UC)患者,8例克罗恩病(Crohn's Disease,CD)患者。结果 (1)在对照组、克罗恩病和溃疡性结肠炎中COX-2的表达分别为:(61.81±20.10),(209.28±9.62)和(200.42±20.33),且对照组与克罗恩病和溃疡性结肠炎组相比,差异有统计学意义(P<0.05);TREM-1在3组中的表达分别为(268.94±4.75),(541.48±153.63)和(487.54±141.59),差异有统计学意义(P<0.05)。TREM-1在UC不同的病情分级及不同内镜分级中表达分别为:病情轻度:(449.55±49.45),中度(499.41±10.81),重度(507.49±67.79),差异有统计学意义(F=19.055,P<0.05);不同内镜分级中的表达是I级:(361.47±55.03),Ⅱ级(481.44±24.28),Ⅲ级(555.01±37.65),差异有统计学意义(F=34.119,P<0.05);COX-2在UC不同的病情分级及不同内镜分级中表达分别为:病情轻度(158.54±15.27),中度(195.25±10.10),重度(233.72±11.43),差异有统计学意义(F=54.617,P<0.05);不同内镜分级中的表达是:Ⅰ级(148.26±16.08),Ⅱ级(203.85±15.43),Ⅲ级(236.35±10.63),差异有统计学意义(F=58.608,P<0.05)。(2)IBD患者结肠黏膜中TREM-1和COX-2的表达呈正相关(r=0.642,P<0.05);UC患者结肠黏膜中TREM-1的表达水平与患者病情程度及内镜分级呈正相关(r=0.945,P<0.05;r=0.944,P<0.05),COX-2的表达水平与患者病情程度及内镜分级亦呈正相关(r=0.944,P<0.05;r=0.944,P<0.05)。结论 TREM-1和COX-2参与了IBD的发病过程,并且可以反映UC的炎症活动情况。Objective The objective of the study was to measure the expression of triggering receptor expressed on myeloid cells-1 （TREM-1） and cyclooxygenase-2 （COX-2） in patients with inflammatory bowel disease （IBD） and to determine their correlations with the severity of ulcerative colitis （UC） and endoscopic classification. Methods Flow cytometry and immunohistochemistry were used to measure the expression of TREM-1 and COX-2 in intestinal mucosa of 60 IBD patients and 30 controls. Among the 60 IBM patients, 52 had ulcerative colitis （UC） and 8 were suffered from Crohn＇s disease （CD）. Results （1） The expressions of COX-2 in the control, CD, and UC groups were 61.81 ＋ 20.10, 209.28 ＋ 9.62, and 200.42 _＋ 20.33, respectively; the differences between the disease groups and the control group were statistically significant （P〈0.05）. The expressions of TREM-1 in the three groups were 268.94 ±4.75, 541.48 ± 153.63, and 487.54 ± 141.59, respectively; the differences were statistically significant （P〈0.05）. The expressions of TREM-1 in different UC severities were 449.55±49.45, 499.41± 10.81, and 507.49 ± 67.79 in mild, moderate, and severe cases, respectively; the differences were statistically significant （F=19.055, P〈0.05）. The expressions of TREM-1 in different endoscopic classifications were 361.47 ± 55.03, 481.44± 24.28, and 555.01 ± 37.65 in grades Ⅰ, Ⅱ, and Ⅲ cases, respectively; the differences were statistically significant （F=34.119, P〈0.05）. The expressions of COX-2 in different UC severities were 158.54 ± 15.27, 195.25 ± 10.10, and 233.72 ± 11.43 in mild, moderate and severe cases, respectively; the differences were statistically significant （F=54.617, P〈0.05）. The expressions of COX-2 in different endoscopic classifications were 148.26± 16.08, 203.85 ± 15.43, and 236.35 ± 10.63 in grades Ⅰ, Ⅱ, and Ⅲ cases, respectively; the differences were statistically significant （F=58.608, P〈0.05）. （2） The expressions of TREM-1

关 键 词：炎症性肠病 髓系细胞表达触发受体-1 环氧化酶-2 

分 类 号：R319[医药卫生—基础医学]