用3种方法检测一起新甲型H1N1型流感病毒感染暴发流行的结果分析  

Result analysis of outbreak of H1N1 influenza virus infection detected by three methods

作　　者：胡晓军[1] 

机构地区：[1]赣州市疾病预防控制中心检验科,江西赣州341000

出　　处：《现代预防医学》2014年第14期2647-2649,2652,共4页Modern Preventive Medicine

摘　　要：目的探讨用3种方法检测2013年5月赣州市某小学暴发的一起急性上呼吸道病毒感染病原学的检测结果。方法采集患者急性期咽拭子标本进行流感病毒核酸检测(荧光PCR);咽拭子标本用MDCK细胞进行病毒分离;用患者急性期和恢复期双份血清进行新甲型H1N1型流感病毒的红细胞凝集抑制试验(HI)。结果 14份咽拭子标本中用荧光PCR扩增检测到了13份新甲型H1N1型流感病毒核酸,阳性率为92.9%。14份咽拭子标本用MDCK细胞分离到10株新甲型H1N1型流感病毒,阳性率为71.4%。14名患者中有9名患者双份血清的新甲型H1N1型流感病毒HI试验恢复期抗体比较急性期抗体有4倍或以上增长(阳性率为64.3%)。结论该次急性上呼吸道感染是由新甲型H1N1型流感病毒引起的,3种检测方法各有特点。Objective This study was conducted to investigate the results from three test methods for a primary outbreak of acute upper respiratory viral infections etiology in Ganzhou, May 2013. Methods Throat swab specimens were collected from patients with acute influenza and were analyzed by influenza virus nucleic acid detection （fluorescent PCR）. Throat swab specimens were pro- cessed for virus isolation using MDCK cells. Sera from acute and recovering patients were analyzed using hemagglutination inhibi- tion test for new type of influenza virus （H1N1）. Results Thirteen of the 14 throat swab specimens （92.9%） tested positive by RT-PCR for New Influenza A H1N1 influenza virus. Ten of the 14 throat swab specimens （71.4%） tested positive by MDCK cell isolation for new influenza A H1N1 influenza virus. Nine of the 14 serum samples （64.3%） had four times or more antibodies against the new influenza A H 1N 1 influenza virus in recovering patients than in acute patients based on the hemagglutination inhibition test. Conclusion The acute upper respiratory tract infections were caused by a new influenza A H1N1 influenza virus and three detection methods have their own characteristics.

关 键 词：新甲型H1N1型流感病毒 聚合酶链反应 病毒细胞分离培养 红细胞凝集抑制试验 

分 类 号：R183.3[医药卫生—流行病学]