精制冠心颗粒复方中4味药材HPLC指纹图谱研究  

Study on HPLC Fingerprint of Four Kinds of Drugs of Jingzhi Guanxin Keli Fufang

作　　者：胡燕 宋力飞 李曼莎 周绍春 肖俊勇 

机构地区：[1]广州白云山奇星药业有限公司,广州510310 [2]广州泽力医药科技有限公司,广州510663

出　　处：《中国现代应用药学》2014年第7期809-813,共5页Chinese Journal of Modern Applied Pharmacy

基　　金："重大新药创制"国家科技重大专项(2011ZX09401-404)

摘　　要：目的利用HPLC建立精制冠心颗粒复方中4味药材指纹图谱。方法运用Agilent 1100 DAD-HPLC高效液相色谱仪,采用Inertsil ODS-3(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.1%磷酸梯度洗脱,流速1.0 mL·min-1,检测波长270 nm,柱温30℃,进样量:10μL,对精制冠心颗粒复方四味药材进行试验。结果 10批精制冠心颗粒复方中4味药材中共获得22个共有峰,不同批次样品相似度均>0.90,方法精密度、稳定性和重复性良好。结论采用HPLC建立的精制冠心颗粒复方中4味药材指纹图谱,方法稳定、可靠、简便,色谱图展现的各峰分离度较好,特征明显,可做为精制冠心颗粒复方真伪鉴别标准。ABSTRACT： OBJECTIVE To establish the chromatographic fingerprint of Jingzhi Guanxin Keli Fufang by HPLC. METHODS HPLC was performed on Jingzhi Guanxin Keli Fufang using Agilent 1100 DAD-HPLC. Inertsil ODS-3 column （4.6 mm×250 mm, 5 μm） was used; the mobile phase was acetonitrile-0.1% phosphoric acid for gradient elution; the flow rate was 1 mL·min-1; the detection wavelength was 270 nm; the column temperature was 30℃, and inject volume was 10μL. RESULTS Twenty-two HPLC common peaks were acquired in the 10 batches of four kinds of drugs of Jingzhi Guanxin Keli Fufang, and their similarities were 〉0.90. Good precision, stability and repetition were showed. CONCLUSION A reliable method is provided for the quality identification of Jingzhi Guanxin Keli Fufang. And the chemical components of different germplasms of Jingzhi Guanxin Keli Fufang are different from each other; selection of germplasm is important in quality control of Jingzhi Guanxin Keli Fufang.

关 键 词：精制冠心颗粒复方 指纹图谱 HPLC 丹参酮ⅡA 

分 类 号：R917.101[医药卫生—药物分析学] R282.5[医药卫生—药学]