抗虫基因Cry1C*重组表达载体的构建与鉴定  

Construction and Identification of Recombinant Expression Vector Containing Insect-Resistant Gene Cry1C*

作　　者：张丽萍[1] 查仁明[2] 李尚伟[1] 

机构地区：[1]贵州大学昆虫研究所,贵州山地农业病虫害重点实验室,贵州贵阳550025 [2]贵州大学农学院,贵州贵阳550025

出　　处：《贵州农业科学》2014年第6期6-9,共4页Guizhou Agricultural Sciences

基　　金：贵州省科技计划课题"转Bt-pta双价抗虫基因水稻的培育及评价"[黔科合NY字(2011)3007]

摘　　要：为水稻害虫生物防治提供菌株,从GenBank中选定Cry1C类抗虫基因,将其优化改造后命名为Cry1C*,Cry1C*经BamH I和Spe I双酶切后与植物表达载体pTCK303连接,构建pTCK303-Cry1C*重组表达载体,将其转化到农杆菌LBA4404中,同时进行PCR、双酶切及测序鉴定。结果表明:PCR、双酶切及测序产物与预期扩增片段(2 342bp)相符,重组表达载体pTCK303-Cry1C*构建成功;农杆菌LBA4404中含有重组质粒,成功构建了Cry1C*农杆菌基因工程菌株。To provide strains for biological control of rice pests,the insect-resistant gene Cry 1C from GenBank was optimized,added BamH I and Spe I restriction enzyme cutting sites at both sides,and artificially synthesized.The modified Bt gene was named Cry 1C ＊ ,which was inserted into pTCK303 after double restriction-enzyme digestion to construct recombinant expression vector pTCK303-Cry 1C ＊ ,and transferred into A.tumefaciens LBA4404.The PCR,double digestion,and sequencing results showed that the recombinant vector was successfully constructed（2 342 bp）.The PCR result showed that the expression vector pTCK303-Cry 1C ＊ had been transformed into A.tumefaciens LBA4404,successfully constructing a genetic engineering bacteria.

关 键 词：水稻 抗虫基因 Cry1C＊ 表达载体构建 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治]