大鲵烂鳃病病原菌的分离鉴定及药敏试验  

Isolation and Identification of Pathogenic Bacteria of Gill Rot Disease from Andrias davidianus and Drug Sensitivity Test

作　　者：贺胜英[1] 叶华[2] 游玲 

机构地区：[1]宜宾学院实验与教学资源管理中心,四川宜宾644007 [2]宜宾学院生命科学与食品工程学院,四川宜宾644007 [3]发酵资源与应用四川省高校重点实验室,四川宜宾644007

出　　处：《贵州农业科学》2014年第6期109-112,共4页Guizhou Agricultural Sciences

摘　　要：为了确定导致大鲵(Andrias davidianus)烂鳃病的主要病原菌类型,从而为大鲵烂鳃病的确诊和科学防治提供参考,从细菌性感染病鲵的鳃、肝、肾及血液中分离到两株细菌,经形态结构、生理生化特征鉴定、16SrDNA基因序列分析鉴定为蜡样芽孢杆菌(Bacillus cereus)和栗褐芽孢杆菌(Bacillus badius)。人工感染试验证实栗褐芽孢杆菌(Bacillus badius)为主要致病菌。两种病原菌对恩诺沙星和环丙沙星比较敏感,可作为首选药物治疗该病。The morphological structure and physiological and biochemical property of two trains isolated from gill,liver,kidney and blood of A.davidianus infected with gill rot disease were identified to determine main pathogenic bacteria types of A.davidianus gill rot disease and to provide a reference for diagnosis and scientific control of A.davidianus gill rot disease.Two stains were identified as B.cereus and Bacillus badius based on 16S rDNA gene sequencing analysis.The artificial infection test confirms that Bacillus cereus is the dominant pathogenic bacteria.Enrofloxacin and CPFX can be used as first choice drugs to control gill rot disease in artificial breeding of A.davidianus.

关 键 词：大鲵 烂鳃病 病原菌 分离鉴定 药敏性 

分 类 号：S852.61[农业科学—基础兽医学]