基于电聚合的苯巴比妥分子印迹电化学传感器的制备及其应用研究  

Preparation and Application of Phenobarbital Electrochemical Sensor Based on Molecular Imprinted Technique and Electropolymerization Membrane

作　　者：余会成[1] 黄学艺[1] 谭学才[1] 雷福厚 毛慧娟[1] 

机构地区：[1]广西民族大学化学化工学院广西林产化学与工程重点实验室,广西南宁530006

出　　处：《分析测试学报》2014年第7期752-757,共6页Journal of Instrumental Analysis

基　　金：广西自然科学重点基金(2013GXNSFDA019006);广西教育厅科研项目(2013YB068);广西研究生教育创新计划资助项目(YCSZ2014120);广西民族大学重点科研项目(2012MDZD040)

摘　　要：以马来松香丙烯酸乙二醇酯（EGMRA）为交联剂，甲基丙烯酸为功能单体，在玻碳电极表面电聚合了一种对苯巴比妥分子具有专一性识别的聚合膜。在最佳实验条件下，采用循环伏安法（CV）、差分脉冲伏安法（DPV）及电化学交流阻抗法（EIS）对印迹传感器的性能进行研究。实验结果表明：该印迹传感器对苯巴比妥具有快速响应、专一性识别和高灵敏度的特点，且苯巴比妥的浓度在8．0×10^-7-1．0×10^-4mol／L范围内与DPV电流信号呈良好的线性关系，相关系数（r）为0．9983。检出限（S／N=3）为5．4×10-mol／L。将此印迹传感器应用于实际样品中苯巴比妥的检测，加标回收率为95．7％～105．0％。A specific molecularly imprinted polymer（MIP） film for the determination of phenobarbital （PB） was electropolymerized on a glassy carbon electrode using methacrylic acid as functional mono- mer and ethylene glycol maleic rosinate acrylate（EGMRA） as cross-linking agent. The optimum ex- perimental conditions were explored. Under the optimum experimental conditions, the response per- formances of the sensor for phenobarbital recognition were investigated by cyclic vohammetry （ CV）, differential pulse voltammetry（DPV） and electrochemical impedance spectroscopy（EIS）. The results showed that the molecularly imprinted film exhibits a quick response, good sensitivity and selectivity to the template molecule phenobarbital. A good linear relationship between DPV current signals and phenobarbital concentration in the range of 8.0 × 10^ -7 - 1.0×10^ -4 mol/L was obtained, with a correlation coefficient（r） of 0. 998 3 and a detection limit（ S/N = 3 ） of 5.4 × 10^ -8 mol/L. The prepared sensor was successfully applied in the determination of phenobarbital in practical samples with recoveries of 95.7% - 105.0%.

关 键 词：苯巴比妥 马来松香丙烯酸乙二醇酯 电聚合 电化学传感器 分子印迹 

分 类 号：O657.1[理学—分析化学] TQ460.72[理学—化学]