盐酸可卡因与对磺化杯[4]芳烃相互作用的研究  

Study on Interaction of p-Sulfonated Calix[4]arene with Cocaine Hydrochloride by Spectrofluorometry

作　　者：杨颖超[1,2] 刘海龙[1] 赵艳[2] 杜黎明[1] 庞涛涛[1] 付云龙[2] 

机构地区：[1]山西师范大学分析测试中心,山西临汾041004 [2]山西师范大学化学与材料科学学院,山西临汾041004

出　　处：《分析测试学报》2014年第7期825-829,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金(21171110);教育部高等学校博士学科点专项科研基金(20091404110001);山西师范大学基金(280001);山西省研究生创新基金(104053)

摘　　要：采用荧光光谱法和核磁共振技术研究了对磺化杯[4]芳烃(SC4A)与盐酸可卡因(CH)间的超分子作用。研究了pH值、温度、离子强度和SC4A浓度对主客体超分子作用的影响。在pH7.5的Britton-Robinson缓冲溶液中,CH的荧光强度随着SC4A的加入而呈下降趋势,荧光的猝灭反映了CH-SC4A包合物的形成。通过光谱滴定法验证了主客体化合物的化学计量之比为1∶1。根据线性方程计算出CH-SC4A包合物的结合常数为6.93×104L/mol。1H NMR研究证明CH中含N—CH3的桥环部分局部进入SC4A的疏水空腔。密度泛函理论计算验证了这一作用机理。The inclusion interaction between＇ cocaine hydrochloride （CH） and p-sulfonated calix [ 4 ] arene（SC4A） was investigated by fluorescence and ^1H NMR spectroscopy. Influences of pH value, temperature, ionic strength and SC4A concentration were examined in detail. In pH 7.5 Britton - Robinson solution, the fluorescence of CH was dramatically reduced to quench upon the addition of SC4A, revealing the formation of inclusion complexes between CH and SC4A. The stoichiometric ra- tio was verified to be 1 ： 1 by the continuous variation method. The binding constant of CH - SC4A complexes was calculated to be 6.93 ~ 104 L/mol by the nonlinear curve fitting method. ^1H NMR spectra showed that the bridge ring moiety containing N--CH3 of CH may partially penetrate into the hydrophobic cavity of SC4A. This was confirmed by density functional theory calculations.

关 键 词：盐酸可卡因 芳烃 荧光分光光度法 密度泛函理论 ^1H NMR技术 

分 类 号：O657.3[理学—分析化学] O629.3[理学—化学]