不同来源肠致病性大肠杆菌(EPEC)分离株eae基因分析  

Typing of intimin(eae) genes from enteropathogenic Escherichia coli strains isolated from human beings and different animal species

作　　者：孙晖[1] 赵爱兰[1] 白向宁[1] 熊衍文[1] 许彦梅[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《实用预防医学》2014年第7期773-775,772,共4页Practical Preventive Medicine

基　　金：国家"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(No.2013ZX10004-001)

摘　　要：目的了解我国不同来源肠致病性大肠杆菌分离株携带的eae基因型别。方法 PCR扩增eae基因,扩增产物测序后在NCBI中进行BLASTn搜索比对,确定其基因型别,并用最大似然法构建eae基因进化树,分析eae基因各型别间的相互关系。结果 130株EPEC菌株中,100株获得了1.8 kb序列,共分为18种eae基因型别,其中以β1型为主,占37%(含37株菌)。人源和动物源EPEC菌株存在相同的eae型别。结论我国EPEC分离株的eae型别呈多样性。Objective To determine the subtypes of eae in enteropathogenic Escherichia coli （EPEC） strains isolated from hu- man cases and animals in China. Methods The eae genes were amplified by PCR, and then the products were sequenced and analyzed with BLASTn. Phylogenetic analysis was conducted using MEGA 5.05. Results Among 130 EPEC strains, the 1.8 kb nucleotide sequences of eae genes of 100 EPEC strains were determined. Eighteen different intimin types were detected, and 131 was the most frequent （37%, 37/100）. Same eae subtypes were found in EPEC strains isolated from human beings and animals. Conclusions The results demonstrate the genetic diversity among eae genes of human and animal EPEC strains in China.

关 键 词：肠致病性大肠杆菌 eae基因 分型 

分 类 号：R378[医药卫生—病原生物学]