反应温度调控生物催化立体选择性的研究进展  

Research Progress on Stereo-Selectivity of Bio-Catalysis by Reaction Temperature Regulation

作　　者：俞恩光 郑仁朝[1] 郑裕国[1] 

机构地区：[1]浙江工业大学生物工程研究所,浙江杭州310014

出　　处：《微生物学杂志》2014年第3期86-90,共5页Journal of Microbiology

基　　金：国家重点基础发展计划(973)资助项目(2011CB710806)

摘　　要：立体选择性调控是手性生物催化的重要内容。反应温度调节作为一种简单、便捷的立体选择性调控方式已在生物催化制备手性化学品中发挥了重要作用。分析了温度引起酶立体选择性变化的机理,并综述了该方法在脂肪酶、酯酶、醇脱氢酶、青霉素G酰化酶和脱卤酶等酶催化反应中的应用。Regulation of stereo-selectivity is an important content of chiral bio-catalysis. Reaction temperature as a simple and convenient method had played important role in preparing chiral chemicals by bio-catlysis. This paper analyzed the mechanism of stereo-selectivity change of enzyme caused by temperature, and summarized its application on the lipase, esterase, SADH, PGA and dehalogenase and other enzyme catalyzed reactions.

关 键 词：生物催化 温度 立体选择性 调控 

分 类 号：Q55[生物学—生物化学]