基于组合策略的慢性阻塞性肺疾病的差异表达基因的筛选  

Screening of Differentially Expressed Genes of COPD Based on Combined Strategy

作　　者：华琳[1,2] 夏翃[1,2] 周萍[1,2] 安立[3] 

机构地区：[1]首都医科大学生物医学工程学院,北京100069 [2]首都医科大学临床生物力学应用基础研究北京市重点实验室,北京100069 [3]首都医科大学附属北京朝阳医院呼吸医学中心,北京100020

出　　处：《中国医疗设备》2014年第7期133-136,共4页China Medical Devices

基　　金：北京市自然科学基金项目(7142015);北京市教育委员会科技计划面上项目(SQKM201210025008);北京市优秀人才培养资助项目(2012D005018000002);国家自然科学基金项目(31100905);首都医科大学基础临床-合作项目(11JL30;11JL33;12JL75)

摘　　要：目的应用组合策略筛选慢性阻塞性肺疾病(COPD)的差异表达基因。方法采用芯片显著性分析算法(SAM)从GEO数据库中提取COPD差异表达基因,并通过5种基因排序算法对基因进行筛选,并对筛选后的COPD相关基因进行随机扰动分析和GO功能富集分析。结果发现GON4L和P4HB是重要的COPD相关基因。结论组合策略提高了COPD易感基因识别的准确率。Objective To screen differentially expressed genes of chronic obstructive pulmonary disease （COPD） through combined strategy.Methods Significance analysis of microarrays （SAM） was used to extract the differentially expressed genes of COPD from GEO database, which then were screened through five kinds of genetic sort algorithms. Then stochastic disturbance analysis and function enrichment analysis of the screened genes were conducted.Results GON4L and P4HB were important genes related to COPD.Conclusion The recognition accuracy of susceptibility genes of COPD has been improved with the application of combined strategy.

关 键 词：慢性阻塞性肺疾病 差异表达基因 随机扰动分析 GO功能富集分析 基因芯片 

分 类 号：R563.9[医药卫生—呼吸系统]