三甲氧基二苯乙烯对小鼠巨噬细胞产肿瘤坏死因子-α及细胞核因子-κB活性的作用研究  

Effects of 3,5,4'-Trimethoxystilbene on TNF-α Production and NF-κB Activity in Murine Macrophages

作　　者：曾清华[1] 饶慧[1] 高洁生[2] 

机构地区：[1]湖南省人民医院肾内风湿免疫科,湖南长沙410006 [2]中南大学湘雅二医院风湿免疫科,湖南长沙410000

出　　处：《中国药业》2014年第14期12-14,共3页China Pharmaceuticals

摘　　要：目的研究三甲氧基二苯乙烯（BTM）对小鼠巨噬细胞经脂多糖（LPS）诱导后产生肿瘤坏死因子-α（TNF-α）及细胞核因子-κB（NF-κB）活化的调控作用。方法培养RAW246．7小鼠巨噬细胞，加入不同浓度的BTM或白藜芦醇，再加入LPS活化细胞，收集细胞上清液用L929细胞结晶紫染色法检测TNF-α的活性，制作细胞爬片用免疫细胞化学法检测巨噬细胞中NF-κB的表达。结果BTM和白藜芦醇本身对L929细胞无细胞毒性；经不同浓度BTM和白藜芦醇处理后巨噬细胞产生TNF-α的水平及表达NF-κB的阳性率呈剂量依赖性减少，BTM抑制巨噬细胞产生TNF-α及表达NF-κB的能力大于白藜芦醇，巨噬细胞产生TNF-α的活性及NF-κB的阳性细胞率均与药物浓度呈负相关，TNF-α的活性和NF-κB的阳性细胞率呈正相关。结论BTM和白藜芦醇在浓度为5～80μmol／L范围内，呈浓度依赖性地通过抑制NF-κB的活化来抑制TNF-α的产生，且BTM抑制巨噬细胞产生TNF-α和NF-κB活化的能力犬于白藜芦醇。Objective To study the regulating effects of 3,5,4＇-trimethoxystilbene（BTM） on the TNF-α production and the NF-κB activation in murine macrophages activated by LPS. Methods The murine macrophage cell line RAW264. 7 cells were cultured and different concentrations of BTM or resveratrol were added,then LPS activated cells were added. The cellular supernatant was collected for detecting the TNF-α activity by the L929 cell crystal violet staining and the cell climbing slides were prepared for detecting the NF-κB expression in macrophages by immunocytochcmistry method. Results BTM and resveratrol had no cytotoxicity to L929 cells. After processing by different concentrations of BTM and resveratrol, the TNF-α production level and the positive rate of NF-κB expression in macrophages showed the dosedependent decrease. The ability of BTM for inhibiting the TNF-α production level and NF-κB expressing in macrophages was greater than that of resveratrol. The activity of macrophages for producing TNF-α and the positive cell rate of NF-κB were negatively correlated with the drug concentration, while the TNF-α activity was positively correlated with the positive cell rate of NF-κB. Conclusion BTM and resveratrol in the concentration range of 5- 80 μmol/L inhibit TNF-α production with a concentration dependent manner by inhibiting NF-κB activation. The ability of BTM for inhibiting TNF-α production and the NF-κB activation in macrophages is greater than that of resveratrol.

关 键 词：三甲氧基二苯乙烯 白藜芦醇 巨噬细胞 肿瘤坏死因子-Α 核因子-ΚB 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]