芦荟大黄素对人永生化角质形成细胞增殖和凋亡作用研究  

Effect of Aloe Emodin on Proliferation and Apoptosis of HaCaT Cells

作　　者：曾德贵[1] 莫衍石[1] 刘荣丰[1] 杨春燕[1] 林世平[1] 陈小谊[2] 兰柳波[2] 

机构地区：[1]广东省深圳市慢性病防治中心,广东深圳518020 [2]广东医学院生物化学与分子生物学研究所,广东湛江524023

出　　处：《中国药业》2014年第14期41-42,共2页China Pharmaceuticals

基　　金：广东省深圳市科技计划项目;项目编号:201202105

摘　　要：目的研究芦荟大黄素（AE）对人永生化角质形成细胞（HaCaT）的生长抑制效应和诱导细胞凋亡能力，探讨其治疗银屑病的可能机制。方法四甲基偶氮唑蓝（MTT）法检测芦荟大黄素对HaCaT细胞增殖的抑制作用，倒置显微镜观察细胞形态学变化，流式细胞术检测细胞周期变化及凋亡。结果芦荟大黄素质量浓度在20～80μg／mL范围内可抑制HaCaT细胞增殖且呈剂量依赖性；镜下观察到细胞稀疏，生长减慢，细胞形态拉长，细胞被阻滞于S期而凋亡。结论芦荟大黄素能抑制HaCaT细胞的增殖、诱导HaCaT细胞的凋亡。可用于治疗银屑病。Objective To study the growth inhibition effect of aloe emodin（AE） on human immotalized keratinocytes （HaCaT） and its apoptotic induction ability,and to explore its possible mechanism in treating psoriasis. Methods The MTT assay was used to detect the proliferation inhibition effect of aloe emodin on the HaCaT cells. The morphological changes were observed by using the inverted micro- scope. The flow cytometry was used to detect the change of cell cycle and apoptosis. Results AE in the range of 20- 80 μg/mL could inhibit the proliferation of HaCaT cells on a dose- dependent manner. The sparse cells, slowed growth and elongated cell shape were observed under microscopy. And the cell cycle was arrested in the S phase after AE treatment,thus apoptosis occurred. Conclusion Aloe emodin inhibits HaCaT cells proliferation and induces HaCaT cells apoptosis,which can be used in the treatment of psoriasis.

关 键 词：芦荟大黄素 人永生化角质形成细胞 银屑病 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]