益阴消渴滴丸质量标准研究  

Quality Standard of Yiyin Xiaoke Dropping Pills

作　　者：张伏军[1] 须建[2] 尹小玲[1] 

机构地区：[1]重庆三峡中心医院药剂科,重庆404000 [2]重庆医药高等专科学校,重庆401331

出　　处：《中国药业》2014年第14期51-52,共2页China Pharmaceuticals

摘　　要：目的建立益阴消渴滴丸的定性鉴别和含量测定方法。方法用薄层色谱（TLC）法定性鉴别益阴消渴滴丸中的葛根、荔枝核；用高效液相色谱（HPLC）法测定葛根中葛根素的含量，色谱条件为迪马C18柱（250mm×4．6mm，5μm），流动相为甲醇-水（25：75），检测波长为250nm柱温30℃。结果TLC色谱斑点清晰，分离度好，阴性对照无干扰；葛根素进样量在0．1738～1．7380μg范围内与峰面积积分值呈良好线性关系（r=0．9999），平均回收率为98．82％，RSD为0．37％。结论所建立的定性、定量分析方法可用于益阴消渴滴丸的质量控制。Objective To establish the methods of qualitative identification and content determination in Yiyin Xiaoke Dropping Pills. Methods The TLC method was used to qualitatively identify Radix puerariae and Semen litchi; and the content of puerarin was determined by HPLC. The chromatographic parameters was the Diamonsil C18 column（250 mm×4.6mm,5μm）,methanol-water（25：75） as the mobile phase,the detection wavelength of 250 nm and the column temperature was 30 ℃. Results The developed TLC sports were clear with good separation and without negative interference;The linear range of puerarin were 0. 173 8- 1. 738 0μg（r=0.999 9）.The average recovery rate was 98.82%, RSD =0. 37%. Conclusion The established qualitative and quantitative analysis method is applicable for the quality control of Yiyin Xiauke Dropping Pills.

关 键 词：益阴消渴滴丸 质量标准 葛根素 荔枝核 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]