HPLC法测定门冬氨酸钾镁注射液中门冬氨酸的含量  

Determination of aspartic acid in potassium magnesium aspartate injection by HPLC

作　　者：徐三能 张静 

机构地区：[1]安徽金太阳生化药业有限公司,安徽阜阳236000 [2]安徽省阜阳市食品药品检验所,安徽阜阳236015

出　　处：《安徽医药》2014年第8期1443-1445,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立HPLC法测定门冬氨酸钾镁注射液中门冬氨酸的含量。方法采用Inertsil ODS-SP色谱柱（5μm,4.6 mm×250 mm）,流动相为乙腈—30 mmol·L^-1十二烷基硫酸钠（加三乙胺0.85 mL,用磷酸调pH至2.5）（150∶850）,流速为1.0 mL·min^-1,检测波长为210 nm,柱温为40℃。结果门冬氨酸在进样量为2.06-12.36μg范围内线性关系良好（r=0.999 8,n=6）,平均回收率为99.72%,RSD为1.0%。结论该方法操作简便、准确、稳定性好,可作为门冬氨酸钾镁注射液中门冬氨酸的有效检测方法。Objective To establish an HPLC method for determination of aspartic acid in potassium magnesium aspartate injection.Methods Inertsil ODS-SP column（5 μm,4. 6 mm × 250 mm） was used. The mobile phase was acetonitrile-30 mmol·L^-1sodium dodecyl sulfate（plus 0. 85 mL triethylamine,pH adjusted to 2. 5 sing phosphoric acid）（150 ∶ 850） with a flow rate of 1. 0 mL·min^-1. The detection wavelength was at 210nm,with the column temperature of 40℃. Results Aspartic acid had a good linear relation in the range of 2. 06 - 12. 36 μg（r = 0. 999 8,n = 6）. The average recovery（n = 6） was 99. 72%,and RSD was 1. 0%. Conclusions This method is simple,accurate,well stable,which can be used as an effective detection method of potassium magnesium injection.

关 键 词：高效液相色谱法 门冬氨酸 门冬氨酸钾镁注射液 

分 类 号：R927.2[医药卫生—药学] O657.72[理学—分析化学]