HPLC法测定二十五味阿魏胶囊中非法添加卡托普利、氯氮、盐酸异丙嗪、硝苯地平的含量  

HPLC determination of captopril,chlordiazepoxide,promethazine hydrochlorine,nifedipine illegally mixed into twenty-five ferulic capsules

作　　者：查道仁[1] 

机构地区：[1]安徽省安庆市立医院,安徽安庆246003

出　　处：《安徽医药》2014年第9期1653-1655,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的：建立二十五味阿魏胶囊中非法添加卡托普利（ KT）、氯氮（ LD）、盐酸异丙嗪（ YB）、硝苯地平（ XB）的高效液相色谱（HPLC）分析方法。方法采用HPLC法，色谱柱为C18柱（4．6 mm ×250 mm，5μm），流动相为乙腈-0．02％磷酸水溶液梯度洗脱，流速为1．0 mL·min^-1，检测波长为215 nm，柱温小于40℃。结果 KT、LD、YB和XB的线性范围分别为0．01012～2．47μg（r^2＝1），0．01116～1．116μg（r2＝1），0．00993～0．993μg（r^2＝0．9999）和0．00981～0．981μg（r＝1）；平均回收率（n＝6）分别为98．70％，97．19％，95．50％，99．41％；相对标准偏差（RSD）分别为2．86％，2．71％，2．48％和1．98％。结论该方法准确性好，重现性好，可作为二十五味阿魏胶囊中非法添加KT、LD、YB和XB的有效分析方法。Objective To set up a method for determination of captopril ,chlordiazepoxide ,promethazine hydrochlorine ,nifedipine ille-gally mixed into twenty-five ferulic capsules by HPLC.Methods HPLC method was adopted.Inertsil ODS-SP C18 column （4.6 mm ×250 mm,5 μm） was used,with the mobile phase consisting of water containing 0.02% phosphoric acid and acetonitrile in gradient mode.The flow rate was 1.0 mL· min^-1 ,detection wavelength was set at UV 215 nm and column temperature was below 40℃.Results The calibration curve showed good linearity for captopril chlordiazepoxide ,promethazine hydrochlorine and nifedipine in the range of 0.0101 2-2.47 μg（r^2 =1）,0.011 16 -1.116 μg（r^2 =1）,0.009 93-0.993 μg（r^2 =0.999 9） and 0.009 81-0.981 μg（r^2 =1）,respectively.The average recovery rates（n=6） were 98.70%,97.19%,95.50%,and 99.41%,respectively.RSD were 2.86%, 2.71%,2.48%,1.98%, respectively.Conclusions The method is accurate and reproducible ,which can be used for the determina-tion of captopril ,chlordiazepoxide ,promethazine hydrochlorine ,nifedipine illegally mixed into twenty-five ferulic capsules .

关 键 词：HPLC 二十五味阿魏胶囊 卡托普利 氯氮革 盐酸异丙嗪 硝苯地平 

分 类 号：R284.2[医药卫生—中药学] O657.72[医药卫生—中医学]