MTT法检测金黄色萄萄球菌活菌数的研究  

Study of the Viable Count of Staphylococcus aureus by MTT Assay

作　　者：袁琳[1] 宁丹[1] 唐曦阳 李晶[1] 

机构地区：[1]中南民族大学药学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2014年第2期54-56,84,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学中央高校基本科研业务费专项基金资助项目(CZY11009);中南民族大学大学生创新项目(KYCX130513B)

摘　　要：以金黄色葡萄球菌为对象,探讨了MTT法应用于细菌细胞数定量分析时,MTT作用时间、毒性、剂量和DMSO用量等因素对活菌计数的影响.结果表明:当细菌数在105～107个/mL时,吸光度值与细菌数目呈良好的正向线性关系,MTT法可准确检测金黄色葡萄球菌的活菌数量.最佳反应条件为:0.25 mg/mL MTT溶液作用2 h,DMSO为2～2.5倍的菌液体积.To analyze the effect of the action time, toxicity, dosage of MTT and the dosage of DMSO on the quantitative analysis of bacterial cells, Staphylococcus aureus was used to quantify its viable count. The results indicated that the absorbance and the number of bacteria were linearly dependent in the range of 10^5 - 10^7 per milliliter. MTr assay thus could be used to accurately detect the number of living bacteria. The optimum condition is 0.25 mg/mL MTT action for 120 minutes with DMSO dosage 2 - 2.5 folds that of the bacteria solution.

关 键 词：MTT法 金黄色葡萄球菌 活菌计数 

分 类 号：Q93-332[生物学—微生物学]