荧光示踪PLGA纳米粒与HL60细胞间相互作用  

Interaction of PLGA nanoparticles with HL60 cells by fluorescence tracking

作　　者：陆丽[1,2] 吕丰[1,2] 武莉[1,2] 刘天军[1,2] 

机构地区：[1]中国医学科学院北京协和医学院生物医学工程研究所,天津300162 [2]天津市生物医学材料重点实验室

出　　处：《国际生物医学工程杂志》2014年第3期148-150,162,I0006,共5页International Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目（31200732）

摘　　要：目的 纳米粒作为药物载体在临床诊断和治疗中有着广泛的研究和应用,其跨细胞膜进入细胞内部的过程与其生物效应直接相关,研究纳米粒与细胞间相互作用可揭示其相关机制.方法 通过荧光示踪法研究荧光素标记的PLGA纳米粒与HL60细胞间相互作用,采用激光共聚焦显微镜定量分析了纳米粒的入胞进程.结果 PLGA纳米粒与HL60细胞间相互作用具有很强的温度依赖性,其中受体介导的细胞内吞机制在纳米粒的入胞过程中起到了重要作用.结论 PLGA纳米粒与HL60细胞间相互作用的研究为纳米药物的设计和应用提供了一定的理论基础.Objective Nanoparticles are widely investigated and applied in clinical diagnosis and treatment as drug carrier,and their transmembrane process is related to their biological effects.The aim of this study was to investigate the interaction of fluorescein-labeled PLGA nanoparticles and HL60 cells via fluorescence tracking.Methods The transmembrane process of nanoparticles was quantitatively analyzed by laser scanning confocal microscopy.Results The analyzing results showed that the interaction of fluorescein-labeled PLGA nanoparticles and HL60 cells was strongly temperature-dependent.The receptor-mediated endocytosis mechanism played an important role in the transmembrane process for cellular uptake of nanopaticles.Conclusions This study provides a theoretical basis for design and application of nano-medicines.

关 键 词：PLGA纳米粒 荧光示踪 HL60细胞 跨膜过程 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] TB383.1[医药卫生—基础医学]