蜘蛛香环烯醚萜类成分ZXX对肠易激综合征模型大鼠的治疗作用和胃肠激素的影响  

Effects of iridoids constituents of Valerianajatamansi Jones ZXX on gastrointestinal sensitivity and gastrointestinal hormones in rats with irritable bowel syndrome

作　　者：王娟[1] 史瑞瑞[2] 王晶[2] 胡京红[1] 高增平[2] 罗武政[1] 施金钹 徐颖洲[1] 闫兴丽[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《长春中医药大学学报》2014年第3期396-399,共4页Journal of Changchun University of Chinese Medicine

基　　金：国家自然科学基金项目(81073132;81357070);校级自主课题(2013JYBZZXS024)

摘　　要：目的探讨蜘蛛香环烯醚萜类成分ZXX对慢性应激所致肠易激综合征(IBS)模型大鼠的治疗作用及其对胃肠激素的影响。方法实验采用雄性SD大鼠72只,分为空白组、模型组、ZXX高、中、低剂量组(1.2、0.6、0.3 mg/kg)、氟西汀组(2.5 mg/kg)和匹维溴铵组(25 mg/kg)进行研究。采用慢性不可预见性应激加孤养的方法造模,观察ZXX对IBS模型大鼠AWR评分、排便潜伏期和粒数的影响,评价对IBS的治疗作用,用放射免疫法测定各组大鼠血浆和结肠中P物质(SP)、血管活性肽(VIP)和生长抑制素(SS)含量。结果与模型组相比,ZXX高中低剂量均可不同程度降低IBS模型大鼠AWR评分,其大剂量效果更显著;各组大鼠血浆中SS、SP和VIP无统计学意义;ZXX各剂量组对结肠中3种胃肠激素均显现出一定的改善作用。结论 ZXX对IBS模型大鼠有较好的治疗作用,并可改善血浆和结肠中的P物质、血管活性肽和生长抑制素的分泌水平。Objective To investigate the effects of iridoid constituents of Valerianajatamansi Jones ZXX on irritable bowel syndrome （IBS） rats caused by chronic unpredictable mild stress （CUMS） and to explore its effect on gastrointestinal hormones in plasma and colon of IBS model rats.Methods Male SD rats （ n = 72） were randomly divided into 6 groups：control group, model group,ZXX high,medium and low dose group （ 1.2,0.6,0.3 mg/kg）, fluoxetine group （2.5 mg/kg） and thePinaverium Bromide （25 mg/kg） in the study. The model was established by CUMS and independent feeding. AWR scores were used to e- valuate the effects of ZXX on visceral sensitivity of IBS model rats. Radio-immunity was used to assay the contents of substance P （SP）,vasoactive peptide （VIP） and Somatostatin （SS） in colon and plasma.Results Compared with the model group,all of the three doses of ZXX can reduce AWR scores in varying degrees, the high dose of ZXX （ 1.2 mg· kg- 1 ） was especially extraordinary; There was no significantly difference of SS, SP and VIP in plasma of the six groups. In colon, the contents of VIP were in an upward trend while SS and SP were downward. All of the three doses of ZXX could improve the level of the three gastrointestinal hormones but no significantly statistical. Conclusion ZXX has a good effect on the treatment of IBS and could improve the level of substance P, vasoactive peptide and Somatostatinin colon and plasma.

关 键 词：蜘蛛香 ZXX 肠易激综合征 胃肠激素 

分 类 号：R285.8[医药卫生—中药学]