蛋白酶及其活性检测方法研究进展  

Research Progress of Proteases and Proteases Activity Assay Methods

作　　者：徐滕[1] 孙梅好[1] 陈果果[1] 倪逸琳 张静[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《山西农业科学》2014年第7期760-763,767,共5页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31070055)

摘　　要：蛋白酶又称蛋白水解酶,其参与了生物体的多种生物过程。它们不仅能催化底物水解,更能调节蛋白质的定位与活性,调节蛋白质与蛋白质的相互作用,参与胞内信号传导以及新的生物活性分子的生成。目前,蛋白酶的活性分析方法可分为均相检测和分离型检测2种。介绍了蛋白酶的种类、功能及其活性的检测方法。Proteases, also called proteolytic enzymes, are involved in a number of biological processes of organisms： proteins loca- tion and protein-protein interactions activity regulation, signal transduction induced by intraceUular signaling molecules, and generation of new bioactive molecules. Protease is one of the cutting-edge researches in biology. There are two common methods of biochemical analysis to detect protease activity： the homogeneous detection and separation-based detection. This article describes the classification and function of proteases and detection methods for protease activity assay.

关 键 词：蛋白酶 活性 种类 检测方法 

分 类 号：Q55[生物学—生物化学]