溴氰菊酯诱导果蝇细胞毒性及相关基因表达的变化  

Induction of the Cytotoxicity and the Relative Genes Expression Response of Deltamethrin in Drosophila Kc cells

作　　者：焦东旭[1] 李娜[1] 程晨[1] 葛春男 程罗根[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2014年第2期106-111,共6页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省高校优势学科(生物学)建设工程项目;江苏省研究生科研创新计划项目;南京师范大学大学生实践创新训练计划项目

摘　　要：溴氰菊酯(deltamethrin,DM)是一种神经毒剂,由于其高效、低毒而被广泛用于农业害虫的防治.长期大范围不合理的使用导致害虫产生抗药性,但目前其确切的抗药性作用机制仍不十分清楚.本研究对溴氰菊酯细胞毒性的研究显示DM染毒可以使果蝇Kc细胞增殖、细胞形态和细胞活性发生明显变化.较低和较高浓度的DM能明显抑制细胞活性,在15 ppm诱导下果蝇细胞的活性有一定程度的升高.实时荧光定量PCR和免疫印迹分析结果显示,经DM处理后的果蝇Kc细胞中与氧化应激反应和免疫相关的基因的表达量显著上调.本研究对细胞毒性和基因表达差异的检测,为进一步探讨与溴氰菊酯抗药性相关的细胞和分子毒理提供了研究基础.Deltamethrin（ DM） is a kind of nerve agent,and commonly used in controlling crop pests,because of the high efficacy,low toxicity. Widespread and improper use of DM induced insect resistance,but the resistance mechanism is not clear at present. Cytotoxicity analysis reveals that the cells proliferation, cellular morphology and cells viability have significant changes under DM stress. Too Low or too high concentration of DM could obviously inhibit the activity of cells. Under 15 ppm treatment, cell activity was higher. Real-time quantitative PCR and western-blotting analysis demonstrated that the genes associated with oxidative stress and immune responses were induced sharply under DM stress. Our research focuses on the cytotoxicity and gene differential expression,these results can provide basis for the study of molecular toxicology and mechanism of deltamethrin resistance.

关 键 词：溴氰菊酯 细胞毒性 实时荧光定量PCR 免疫印迹分析 

分 类 号：S481.4[农业科学—农药学]