高效液相色谱法测定麻仁胶囊中结合蒽醌的含量  

HPLC determination of Maren capsule combined anthraquinone content

作　　者：张凤羽[1] 刘冰[2] 

机构地区：[1]天津市静海县药品检验所,天津301600 [2]天津市药品检验所,天津300070

出　　处：《天津药学》2014年第3期7-10,共4页Tianjin Pharmacy

摘　　要：目的：建立HPLC法测定麻仁胶囊中结合蒽醌含量的方法。方法：采用高效液相色谱法，色谱柱为Agilent ZORBAX SB—C18（250mm×4．6mm，5μm），以乙腈-甲醇-0．1％磷酸（42：23：35）为流动相，流速为1ml／min。检测波长为254nm。结果：大黄素进样量在0．01～0．20μg范围内，与峰面积线性关系良好（r=0．9999）；大黄酚进样量在0．03027—0．6054μg范围内，与峰面积线性关系良好，（r=0．9999）。总大黄素平均回收率为100．33％，RSD为1．37％（n=6）；总大黄酚平均回收率为99．24％，RSD为2．20％（n=6）。游离大黄素平均回收率为101．76％，RSD为0．71％（n=6）；游离大黄酚平均回收率为101．10％，RSD为1．15％（n=6）。结论：建立的方法操作简便，专属性和重复性好，可用作该制剂的质量控制方法。Objective： To establish a method for determination combined anthraquinone content of Maren capsule. Methods：The chromatographic separation on an Agilent ZORBAX SB - C18 （250 mm × 4. 6mm, 5 μm） column with the mobile phase of aceto- nitrile - methanol - 0. 1% phoric acid solution （42： 23： 35 ）. The flow rate was 1 ml/min,the detection wavelength was set at 254 nm. Results ：The calibration curve of emodin was linear within a range of 0.01 - 0.2μg（ r = 0.999 9 ） ;chrysophanol was linear within a range of 0. 030 27 - 0. 605 4μg （ r = 0. 999 9 ）. Total emodin average recovery was 100.33 %, RSD was 1.37 % （ n = 6 ）. Total chrysophanol average recovery was 99.24% , RSD was 2.20% （ n = 6 ）. The average recovery of free emodin average recovery was 101.76% , RSD was 0.71% （n = 6） ; free chrysophanol average recovery was 101.10%, RSD was 1.15% （ n = 6）. Conclusion ： The method is accurate, senstitvte and specific. It can be used for the quality control of the preparation.

关 键 词：高效液相色谱法 麻仁胶囊 结合蒽醌 含量 大黄素 大黄酚 

分 类 号：R927.2[医药卫生—药学]