冠心病患者白细胞介素8与白细胞血小板间黏附的相关性研究  

Correlation between IL-8 level and platelet-leukocyte adhesion in coronary heart disease patients

作　　者：郭春雨[1] 吴彩凤[1] 马晓娟[1] 刘欣[1] 殷惠军[1] 

机构地区：[1]中国中医科学院西苑医院心血管病中心,北京100091

出　　处：《中华老年心脑血管病杂志》2014年第7期716-719,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81073086;81030063)

摘　　要：目的通过分析冠心病患者血清白细胞介素8(interleukin-8,IL-8)表达量与血小板糖膜蛋白(GP)Ⅱb/Ⅲa活性、白细胞血小板聚集率之间的关系,探讨趋化因子IL-8参与冠心病发病的作用机制。方法选择冠心病患者102例(冠心病组),健康体检者45例(对照组),冠心病组于冠状动脉造影前,空腹抽取静脉血6ml,对照组于清晨空腹抽取静脉血6ml,采用流式细胞术检测白细胞血小板聚集率及GPⅡb/Ⅲa表达水平,应用酶联免疫吸附法检测血清IL-8水平。结果冠心病组IL-8[(65.98±26.44)ng/L vs(41.91±19.67)ng/L]、GPⅡb/Ⅲa[(15.06±7.89)%vs(8.06±2.34)%]、血小板中性粒细胞聚集率和血小板单核细胞聚集率较对照组明显升高,差异有统计学意义(P<0.01)。相关性分析显示,IL-8与血小板中性粒细胞聚集率、血小板单核细胞聚集率和GPⅡb/Ⅲa呈正相关(P<0.05,P<0.01)。结论冠心病患者血清IL-8表达水平与GPⅡb/Ⅲa活性、白细胞血小板聚集率显著相关,可作为潜在的检验指标和治疗靶点进行临床应用推广。Objective To study the role of IL-8 level in pathogenesis of coronary heart disease （CHD） by analyzing its correlation with platelet membrane protein （GP） Ⅱ b/Ⅲ a activity and platelet-leukocyte conjugate rate in CHD patients.Methods One hundred and two CHD patients served as a CHD group and 45 healthy volunteers served as a control group in this study.Fasting blood samples （6 ml） were taken from CHD patients before coronary angiography and healthy volunteers.GP Ⅱ b/Ⅲ a activity and platelet-leukocyte conjugate rate were measured by flow cytometry.Serum IL-8 level was measured by ELISA.Results The serum IL-8 level,GP Ⅱ b/Ⅲ a activity and platelet-leukocyte conjugate rate were significantly higher in CHD group than in control group （P＜0.01）.Correlation analysis showed that the IL-8 level was positively related with the platelet-leukocyte conjugate rate,platelet-monocyte conjugate rate,and GP Ⅱ b/Ⅲ a activity （P＜0.05,P＜0.01）.Conclusion Serum IL-8 level is closely related with GP Ⅱ b/Ⅲ a activity and platelet-leukocyte conjugate rate in CHD patients,and can thus be used as a potential laboratory index and treatment target in clinical practice.

关 键 词：白细胞介素8 冠心病 白细胞 血小板糖蛋白GPⅡb-Ⅲa复合物 血小板粘附 

分 类 号：R541.4[医药卫生—心血管疾病]