冷诱导RNA结合蛋白在亚低温下的神经保护作用研究  

Role of cold-inducible RNA binding protein in protection of neurons under hypothermia

作　　者：蔡英[1] 郑君毅[2] 王冠 武俏丽[1] 巫嘉陵[1] 苏心[1] 

机构地区：[1]天津市环湖医院天津市神经外科研究所天津市脑血管与神经变性重点实验室,300060 [2]天津市胸科医院 [3]天津市中医药大学第二附属医院

出　　处：《中华老年心脑血管病杂志》2014年第7期754-757,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81303091);天津市应用基础与前沿技术研究计划(13JCYBJC23200);天津市卫生局科技基金(2012KR09;2012KZ034)

摘　　要：目的研究亚低温下冷诱导RNA结合蛋白(CIRP)对受损海马神经元的保护作用机制。方法将原代培养的海马神经元分为3组:对照组、损伤组和治疗组。氧糖剥夺55min制作海马神经元损伤模型,损伤组和治疗组在氧糖剥夺55min后分别置于37℃和32℃培养24h。收集各组细胞,MTT检测各组细胞的存活率,荧光定量PCR和Western blot检测CIRP的mRNA和蛋白的表达水平,流式细胞术检测各组细胞凋亡率,Western blot检测Caspase-3蛋白表达水平。结果与对照组比较,损伤组细胞生存率明显下降[(43.3±8.1)%vs(100.0±12.7)%,P<0.05],治疗组细胞生存率明显升高[(71.8±13.6)%vs(43.3±8.1)%,P<0.05],与损伤组比较,治疗组细胞凋亡率下降[(19.5±0.6)%vs(30.7±2.5)%],CIRP的mRNA蛋白表达明显升高,Caspase-3蛋白表达水平降低,差异有统计学意义(P<0.05)。结论亚低温下CIRP表达水平升高,通过抑制细胞凋亡发挥神经元保护作用,是亚低温脑保护作用途径之一。Objective To study the role of cold-inducible RNA binding protein （CIRP） in protection of injured hippoeampal neurons under hypothermia.Methods Primarilycultured hippocampal neurons were divided into control group,injury group and treatment group.A model of injured hippocampal neurons was established by depriving oxygen glucose for 55 min.The hippocampal neurons in injury group and treatment group were cultured for 24 h at 37℃ and 32℃ after depriving oxygen glucose for 55 min.The cell survival rate in different groups was assayed by MTT.The CIRP mRNA and protein expressions were detected by RT-PCR and Western blot,respectively.The apoptosis rate in different groups was measured by flow cytometry.Results The cell survival rate was significantly lower in injury group than in control group and in treatment group than in injury group,and significantly higher in treatment group than in control group （43.3％± 8.1％ vs 100.0％ ± 12.7％,P＜0.05; 19.5％ ±0.6％ vs 30.7％ ± 2.5％,P＜0.05; 71.8％ ± 13.6％ vs 43.3％±8.1％,P＜0.05）.The CIRP mRNA and protein expression levels were significantly higher while the Caspase-3 expression level was significantly lower in treatment group than in injured group （P＜0.05）.Conclusion The high CIRP expression level under hypothermia can protect injured hippocampal neurons by inhibiting cell apoptosis.

关 键 词：低温 RNA结合蛋白质类 海马 神经元 细胞凋亡 

分 类 号：R651.15[医药卫生—外科学]