川中黑山羊(金堂类群)和藏山羊PRLR基因cDNA克隆及序列分析  

Cloning and sequence analysis of PRLR gene in Jintang black goat and Tibetan goat

作　　者：胡亮[1] 字向东[1] 卢建远[1] 马力[1] 熊显荣[1] 王永[1] 任称罗尔日 任陶 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]四川省理县畜牧局,四川理县623100

出　　处：《西南民族大学学报（自然科学版）》2014年第4期485-488,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省应用基础项目(2013JY0043)

摘　　要：根据GenBank中绵羊的PRLR基因序列设计引物,以川中黑山羊(金堂类群)和藏山羊垂体总RNA为模板,通过RT-PCR技术对川中黑山羊(金堂类群)和藏山羊PRLR基因cDNA克隆及进行序列分析,以期为进一步研究该基因与山羊的繁殖性能奠定理论基础.结果表明:川中黑山羊(金堂类群)和藏山羊PRLR基因编码区均长1746bp,均编码581个氨基酸.川中黑山羊(金堂类群)PRLR基因编码区与藏山羊、绵羊、牛、牦牛野猪和人的同源性分别为99.71%、99%、95%、95%、83%和80%.以核苷酸序列构建分子系统进化树,川中黑山羊(金堂类群)先与藏山羊聚为一类,再与绵羊聚为一类,后与牛和牦牛聚为一类,然后与野猪聚为一类,最后与人聚为一类.The primers were designed according to the GenBank gene sequence of sheep PRLR. Total RNA was extracted from the pituitary of Jintang black goat and Tibetan goat. The PRLR gene of Jintang black goat and Tibetan goat were cloned and the sequences were analyzed in order to understand the effect of this gene on reproductive performance in goat. The results showed that the amplification coding region of the PRLR gene in Jintang black and Tibetan goat was 1746bp long, encoding 518 amino acids. The homologies of CDS ofPRLR gene between Jintang black goat and Tibetan goat, sheep, cattle, yak, pig and human were 99.71%, 99%, 95%, 95%,83% and 80%. Phylograms constructed on the basis of CDS from species indicated that Jintang black goat and Tibetan goat, then assembled with sheep, cattle sand yak assembled separately, and then assembled with pig, and then assembled with human finally.

关 键 词：川中黑山羊(金堂类群) 藏山羊 PRLR基因 克隆 序列分析 

分 类 号：S814[农业科学—畜牧学] S827[农业科学—畜牧兽医]