双峰驼肝脏CYP3A亚酶活性的初步研究  

Preliminary Studies on CYP3A Sub-enzyme Activities of Bactrian Camel

作　　者：王学恩[1] 裴乐[2] 格日勒图亚 哈斯苏荣[2] 

机构地区：[1]阜新高等专科学校农牧系,辽宁阜新123000 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [3]内蒙古乌审旗兽医局,内蒙古乌审旗017300

出　　处：《畜牧与饲料科学》2014年第6期22-24,共3页Animal Husbandry and Feed Science

基　　金：国家自然科学基金项目(31260623)

摘　　要：CYP3A亚酶参与45％～60％常用药物的生物转化，在机体处置外源性物质的过程中发挥着重要作用。为了探讨双峰驼肝脏CYP3A亚酶活性，采用改进的钙离子沉淀法制备肝微粒悬液，并借助生物化学方法对双峰驼CYP3A亚酶活性进行了初步研究。结果表明，钙离子沉淀法制备的双峰驼肝微粒体蛋白含量为（1．541±0．264）mg／mL，CYP总酶含量为（0．412±0．063）nmol／mg，红霉素-N-脱甲基酶的活性为（1．159±0．001）nmol／（mg·min）。以上研究结果为今后借助探针药物检测双峰驼CYP3A亚酶的体外代谢活性的深入研究奠定了基础。CYP3A sub-enzymes participate in biotransformation of 45%-60% of commonly used drugs, and play an important role in regulating the interaction of body and external environment and maintaining homeostasis. In order to study the activity of hepatic CYP3A sub-enzymes, the hepatic microsome was prepared by the Ca^2＋ precipitation method, and the activities of CYP3A sub-enzyme was preliminarily studied by using biochemical methods. The result showed that the protein content of hepatic microsome was （1.541±0.264） mg/mL, the content of total CYP was （0.412±0.063 ） nmol/mg, and the activity of erythromycin-N- demethylase was （1.159±0.001） nmol/（mg· min ）, respectively. The results laid a good foundation for further research of metabolic activity of CYP3A subfamily in vitro by using probe drug detection.

关 键 词：双峰驼 肝微粒体 CYP3A亚酶活性 

分 类 号：R965.2[医药卫生—药理学] S824[医药卫生—药学]