吞噬凋亡淋巴细胞的树突状细胞诱导高分泌白细胞介素-10的调节性B细胞的生成  

Dendritic cells phagocytized apoptotic lymphocytes induce the development of regulatory B cells with high secretion of interleukin-10

作　　者：魏玉香[1] 王晓刚[1] 肖漓[1] 周文强[1] 郑德华[1] 于涛[1] 石炳毅[1] 

机构地区：[1]解放军第309医院全军器官移植研究所器官移植与免疫调节北京市重点实验室,北京100091

出　　处：《器官移植》2014年第4期237-241,共5页Organ Transplantation

基　　金：国家自然科学基金(81370578)

摘　　要：目的探讨未成熟树突状细胞(imDC)吞噬经补骨脂素长波紫外线(PUVA)处理的小鼠脾B淋巴细胞(PUVA-SP)后,将B淋巴细胞诱导为高分泌白细胞介素(IL)-10(IL-10+)的调节性B细胞的可行性。方法培养小鼠骨髓来源DC,小鼠脾淋巴细胞经PUVA光源照射得到PUVA-SP,在体外将PUVA-SP与骨髓来源的imDC共同培养,后者吞噬PUVA-SP后形成PUVA-SP DC。应用CD19磁珠分选小鼠脾脏B淋巴细胞,将不同的DC与B淋巴细胞共同培养48 h。采用酶链免疫吸附试验(ELISA)检测B淋巴细胞、imDC、imDC+B淋巴细胞、PUVA-SP DC、PUVA-SP DC+B淋巴细胞的培养上清液中干扰素(IFN)-γ、转化生长因子(TGF)-β、IL-12p70、IL-10等细胞因子的含量。流式细胞仪分析B淋巴细胞、imDC+B淋巴细胞、mDC+B淋巴细胞、PUVA-SP DC+B淋巴细胞共培养后IL-10+调节性B细胞数量。结果与其他4组细胞培养液比较,PUVA-SP DC+B淋巴细胞上清液中的IL-10含量明显升高(均为P<0.05)。与其他各组相比,PUVA-SP DC+B淋巴细胞中IL-10+调节性B细胞数量明显增多。结论 PUVA-SP DC可以诱导脾脏B淋巴细胞分化为IL-10+调节性B细胞。Objective To investigate the feasibility of immature dendritic cells （imDC）phagocytized psoralen ultraviolet A （PUVA）-treated splenic lymphocytes （PUVA-SP DC）in mice inducing B lymphocytes to be regulatory B cells （Breg）with high secretion of interleukin （IL）-10 （IL-10 ＋Breg）. Methods Bone marrow-derived DC of mice was cultured. Spleen lymphocytes of mice were isolated and treated by PUVA,and turned to be PUVA-SP. The bone marrow-derived imDC was co-cultured with PUVA-SP in vitro to obtain PUVA-SP DC. Splenic B lymphocytes of mice were separated by anti-CD19 magnetic beads and co-cultured with different kinds of DC for 48 hours. The levels of interferon （IFN ）-γ,transforming growth factor （TGF）-β,IL-12p70,and IL-10 in the culture supernatant of B lymphocytes,imDC,imDC＋B lymphocytes, PUVA-SP DC and PUVA-SP DC ＋B lymphocytes were measured by enzyme-linked immune absorbent assay （ELISA）. The accounts of IL-10 ＋Breg in B lymphocytes,imDC＋B lymphocytes,mDC＋B lymphocytes and PUVA-SP DC＋B lymphocytes were detected by flow cytometry. Results Compared with the other 4 groups, the level of IL-10 in cell culture supernatant of PUVA-SP DC＋B lymphocytes was significantly higher （all in P〈0.05）. Compared with the other groups,the account of IL-10 ＋Breg in PUVA-SP DC＋B lymphocytes was＆amp;nbsp;significantly higher. Conclusions PUVA-SP DC can induce splenic B lymphocytes to differentiate into IL-10 ＋Breg.

关 键 词：树突状细胞 体外光化学方法 调节性B细胞 白细胞介素-10 

分 类 号：R617[医药卫生—外科学]