氯酸钾-甲苯胺蓝催化褪色光度法测定痕量亚硝酸根的研究  

Catalytic Spectrophotometric Determination of Trace Nitrit Based on the Oixidation of Toluidine blue by Potassium Chlorate

作　　者：刘秀萍[1] 

机构地区：[1]晋中学院化学化工学院,山西晋中030600

出　　处：《海南师范大学学报（自然科学版）》2014年第2期157-159,共3页Journal of Hainan Normal University（Natural Science）

摘　　要：基于6 mol/L乙酸介质中,亚硝酸根对KClO3氧化甲苯胺蓝的褪色反应具有明显的催化作用,建立了测定痕量亚硝酸根的新的催化光度分析法,研究了反应的适宜条件及动力学参数.在最大吸收波长628 nm及选择的实验条件下,线性范围为0.05～0.4μg/mL,检出限为4.1×10-3μg/mL.该方法在80℃水浴加热,操作方便,灵敏度高,选择性好,大多数离子不干扰,且稳定,可直接用于雨水及唾液样品中痕量亚硝酸根的测定,结果满意.A new catalytic spectrophotometric method for determining trace nitrite was developed. It is based on the cata- lytic effect of trace nitrite on the oxidation of Toluidine blue by potassium Chlorate in acetic acid. This method was operat- ed at 80℃ and without disturbance from many ions. The optimum experiment condition for the determination was found. Ki- netic parameters were studied. The detection limit for nitrite is 4.1×10-3 μ/mL, the linear range of the determination is 0.05- 0.4 μg/mL. The method is sensitive and selective. The method can be applied for the determination of trace nitrite in rain water and saliva samples with satisfactory results.

关 键 词：催化光度法 氯酸钾 甲苯胺蓝 亚硝酸根 

分 类 号：O657.3[理学—分析化学]