泉州市6例布鲁菌病的微生物生化鉴定及分子生物学确认  

Comparative Identification between Biochemical and Molecular Biological Method of Six Strains of Brucellosis in Quanzhou City

作　　者：黄东红[1] 郑友限 潘少敏[1] 余晓完 

机构地区：[1]福建医科大学附属第二医院检验科,泉州362000 [2]泉州市疾病控制中心检验科,泉州362000

出　　处：《福建医科大学学报》2014年第2期125-127,共3页Journal of Fujian Medical University

摘　　要：目的验证布鲁菌的微生物鉴定和PCR检测结果的一致性。方法利用全自动细菌鉴定仪对血(骨髓)培养阳性的标本进行微生物鉴定,提取细菌DNA,应用布鲁菌通用引物BCSP31进行PCR扩增,产物进行测序。结果 6例培养阳性的病原菌经鉴定为布鲁菌。经荧光PCR检测、测序结果在线blast分析确认为布鲁菌。结论细菌的分离培养结合PCR方法可以提高布鲁菌的检测。Objective To compare the conformity between biochemical identification and PCR detection technology of Brucella.Methods The blood （marrow） positive samples were identified by VITECK 2 COMPACT,while extraction of microbial DNA was amplified using Brucella universal primersBCSP31.The PCR results were examined with agarose gel electrophoresis and sequenced.Results The six pathogenic bacteria were identified as Brucella by VITECK 2 COMPACT,and they were also detected conformity by fluorescent PCR and by online blast analysis.Conclusion Combination of isolation and cultivation of bacteria with BCSP31 PCR method can improve the detection of Brucella.

关 键 词：布鲁杆菌病 布鲁杆菌属 传染病 基因 细菌 分子生物学 聚合酶链反应 微生物敏感性试验 福建 

分 类 号：R516.7[医药卫生—内科学] R446.5[医药卫生—临床医学]