hsa-miR-26b表达沉默对脂肪细胞蛋白分泌的影响  

Effect of hsa-miR-26b-silenced lentivirus on secretion of adipocytokines

作　　者：杨蕾[1] 徐广峰[2] 季晨博[1] 史春梅[1] 陈玲[1] 庞玲霞[1] 赵亚萍[2] 郭锡熔[1] 

机构地区：[1]南京医科大学儿科研究所,江苏省210029 [2]中国人民解放军第八二医院检验科

出　　处：《江苏医药》2014年第13期1489-1491,F0002,共4页Jiangsu Medical Journal

基　　金：国家重点基础研究发展计划(2013CB530604);国家自然科学基金(81170797);江苏省自然科学基金(BK2011107);江苏省医学创新团队项目(LJ201108);南京市科技发展计划(201104013);蚌埠医学院科研课题项目(Byky1262NF);军区医学科技创新课题项目(12MA025)

摘　　要：目的探讨hsa-miR-26b表达沉默对脂肪细胞蛋白分泌的影响。方法将hsa-miR-26b表达沉默组及对照组慢病毒感染人脂肪前体细胞,在诱导分化过程中收集不同时间点的细胞培养基上清液,采用ELISA法检测IL-6和瘦素(leptin)的分泌量。结果在细胞分化过程中,hsa-miR-26b表达沉默组IL-6与leptin表达量均较对照组降低(P<0.01或P<0.05)。结论降低hsa-miR-26b表达在一定程度上可以改善人脂肪细胞炎症状态及胰岛素抵抗,为肥胖治疗提供潜在靶点。Objective To investigate the effect of hsa-miR-26b-silenced lentivirus on the secretion of adipocytokines during differentiation.Methods Human preadipocytes were infected with hsa-miR-26b-silenced lentivirus（group A）or control lentivirus（group B）.The supernatant of culture medium was collected at different time points of differentiation,and the secretions of IL-6and leptin were detected by ELISA.Results Compared with group B,the expressions of IL-6and leptin were decreased during differentiation in group A（P〈0.01or P〈0.05）.Conclusion Downregulation of hsa-miR-26bexpression can improve the inflammation and insulin resistance of human adipocytes,which may provide a potential target for the treatment of obesity.

关 键 词：hsa-miR-26b 脂肪细胞 脂肪细胞蛋白 肥胖 

分 类 号：R725[医药卫生—儿科]