地塞米松促进人甲状腺滤泡上皮原代细胞凋亡  

Dexamethasone promotes apoptosis of primary human thyroid follicular epithelial cells

作　　者：何珂[1] 胡蕴[1] 郭慕红[2] 张斌[2] 姜鹏[2] 毛晓明[1] 

机构地区：[1]南京医科大学附属南京医院南京市第一医院内分泌科,江苏南京210012 [2]南京医科大学附属南京医院南京市第一医院普外科,江苏南京210012

出　　处：《基础医学与临床》2014年第7期950-954,共5页Basic and Clinical Medicine

基　　金：南京市医学科技发展项目(ZKX12023)

摘　　要：目的 研究地塞米松对人甲状腺滤泡上皮细胞凋亡的影响及其临床意义.方法 分离原代甲状腺滤泡上皮细胞,以0、10-6、10-5和10-4mol/L地塞米松刺激单层培养的甲状腺细胞,用MTT、流式细胞术及荧光定量PCR法检测刺激前后甲状腺细胞的增殖抑制率、凋亡率及凋亡相关基因mRNA的表达.结果 地塞米松在0～10-4mol/L浓度范围内可剂量依赖性地抑制甲状腺细胞增殖（P＜0.05）,增加甲状腺细胞凋亡率（P＜0.05）及凋亡相关基因mRNA的表达（P＜0.01）.结论 地塞米松可抑制甲状腺细胞增殖,促进甲状腺细胞凋亡的发生.Objective To investigate the effect of dexamethasone on apoptosis of primary human thyroid follicular epithelial cells.Methods After separation and stimulation with different concentrations of dexamethasone,inhibition of proliferation,apoptosis and apoptosis-related mRNA expression of primary thyroid follicular epithelial cells were measured by MTT,flow cytometry and fluorescence quantitative PCR respectively.Results Within the concentration range of 10-6 ～ 10-4 mol/L,dexamethasone dose-dependently inhibited thyroid cell proliferation,increased cell apoptosis and expression of apoptosis-related mRNA of thyroid cells.Conclusions Dexamethasone can not only inhibit the proliferation of thyroid follicular epithelial cells,but also have effect on apoptosis of thyroid cells.

关 键 词：地塞米松 甲状腺滤泡上皮细胞 细胞增殖 细胞凋亡 

分 类 号：R581.1[医药卫生—内分泌]