2036例过敏相关疾病体外过敏原特异性IgE检测与分析  

In vitro detection and analysis of allergen-specific IgE antibodies in 2 036 patients with allergic diseases

作　　者：李慧[1] 冯海瑕 俞先水 王璐[1] 刘玉峰[1] 李巍[1] 

机构地区：[1]第四军医大学西京皮肤医院,西安710032

出　　处：《国际皮肤性病学杂志》2014年第4期212-214,共3页International Journal of Dermatology and Venereology

基　　金：国家自然科学基金（81273320）

摘　　要：目的 分析过敏相关疾病体外过敏原特异性IgE（sIgE）的阳性检出率及与临床的相关性.方法 门诊特应性皮炎、湿疹、荨麻疹等过敏性皮肤病患者,应用免疫印迹法检测sIgE,用自制调查问卷分析阳性sIgE与临床症状的相关性.结果 对2 178例受试者（特应性皮炎163例,湿疹268例,荨麻疹1 522例,过敏性鼻炎61例,过敏性哮喘22例,健康者142例）的检测结果进行分析,发现特应性皮炎患者sIgE≥2级和≥3级的阳性率分别为54.60％和38.04％,显著高于湿疹（35.07％和20.15％）和荨麻疹（36.47％和19.05％）（P＜ 0.001）;特应性皮炎过敏原sIgE3级和≥4级时与患者临床症状的相关性较高（61.11％和83.05％）,显著高于湿疹（9.61％和14.63％）和荨麻疹（7.36％和15.43％）,差异有统计学意义（P＜ 0.001）.特应性皮炎患者常见的过敏原为尘螨（15.95％）、艾蒿（11.04％）、猫毛（7.36％）、蛋清（6.14％）、屋尘（5.52％）.结论 过敏原sIgE检测在特应性皮炎的临床应用价值高于湿疹和荨麻疹.Objective To detect allergen-specific IgE （sIgE）antibodies in patients with allergic diseases and to assess their clinical relevance.Methods Serum samples were collected from 2 036 outpatients with allergic diseases （including 163 cases of atopic dermatitis,268 eczema,1 522 urticaria,61 allergic rhinitis,22 allergic asthma） as well as 142 healthy human controls.Immunoblotting was performed to detect sIgE antibodies in these samples.A survey was conducted with a self-designed questionnaire to assess clinical reactivity to tested allergens in these subjects.Results The proportions of patients with a sIgE score of grade ≥ 2 and those with a sIgE score of grade ≥ 3 were 54.60％ and 38.04％ in patients with atopic dermatitis,respectively,significantly higher than those in patients with eczema （35.07％ and 20.15％,respectively,both P ＜ 0.001）and patients with urticaria （36.47％ and 19.05％,respectively,both P ＜ 0.001）.The coincidence rate between sIgE detection results and self-reported allergy was 61.11％ and 83.05％ in atopic dermatitis patients with a sIgE score of grade 3 and grade ≥ 4,respectively,significantly higher than that in eczema patients （9.61％ and 14.63％,respectively,both P ＜ 0.001） and urticaria patients （7.36％ and 15.43％,respectively,both P ＜ 0.001）.The five most common allergens were dust mite （15.95％）,Artemisia argyi （11.04％）,cat hair （7.36％）,egg white （6.14％） and house dust （5.52％）among patients with atopic dermatitis.Conclusion sIgE detection appears to be of higher clinical value to patients with atopic dermatitis than to those with eczema or urticaria.

关 键 词：皮肤病学 过敏反应 免疫球蛋白E 变应原 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]