NF-κB受体活化因子配体在假体无菌性松动患者外周血中的表达  

EXPRESSION OF RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-κB LIGAND IN PERIPHERAL BLOOD OF PATIENTS WITH ASEPTIC LOOSENING OF THE IMPLANT AFTER TOTAL HIP APTHROPLASTY

作　　者：侯开宇[1] 王宇飞[1] 陆晓涛[1] 杨克敏[1] 孔维云[1] 李勇刚[1] 

机构地区：[1]云南省第二人民医院骨科,昆明650031

出　　处：《中国修复重建外科杂志》2014年第7期853-856,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的通过与正常髋关节患者比较,探讨人工全髋关节置换术(total hip arthroplasty,THA)后假体无菌性松动患者外周血中NF-κB受体活化因子配体(receptor activator of NF-κB ligand,RANKL)的表达变化,分析其与假体无菌性松动间的关系。方法以2008年1月-2013年1月58例THA术后假体无菌性松动患者作为试验组,63例股骨颈骨折患者作为对照组。两组患者年龄及性别比较,差异无统计学意义(P>0.05)。取两组患者清晨空腹抗凝外周血,采用荧光定量PCR、Western blot及ELISA法检测RANKL基因、蛋白表达水平及其浓度。结果试验组RANKL基因、蛋白相对表达量及其浓度分别为18.30±1.09、0.856±0.254、(3.553 5±0.129 7)ng/mL,显著高于对照组的1.00±0.05、0.404±0.102、(1.912 3±0.126 2)ng/mL,比较差异均有统计学意义(t=125.390,P=0.000;t=13.032,P=0.000;t=18.124,P=0.000)。结论外周血中RANKL可能是THA术后假体无菌性松动的预测因子。Objective To investigate the expression changes of the receptor activator of nuclear factor-κB ligand（RANKL） in the peripheral blood of patients with aseptic loosening of the implant after total hip arthroplasty（THA） by comparing with that of patients with femoral neck fracture and to analyze the correlation between RANKL expression and aseptic loosening. Methods Between January 2008 and January 2013, the peripheral blood were harvested from 58 patients with aseptic loosening of the implant after THA（trial group） and 63 patients with femoral neck fracture（control group）. The 2 groups were well matched, with no signii cant dif erences in age and gender（P 0.05）. The expressions of the RANKL mRNA and RANKL protein were evaluated by quantitative real-time PCR and Western blot respectively. At the same time, the concentration of RANKL was also measured by ELISA. Results The expression of the RANKL mRNA in the trial group was 18.30 ± 1.09, which was signii cantly higher than that of control group（1.00 ± 0.05）（t=125.390, P=0.000）. The relative RANKL protein expression values in trial group and control group were 0.856 ± 0.254 and 0.404 ± 0.102 respectively, showing signii cant dif erence（t=13.032, P=0.000）. The results of ELISA showed that the concentration of RANKL in trial group [（3.553 5 ± 0.129 7） ng/mL] was signii cantly higher than that of control group [（1.912 3 ± 0.126 2） ng/mL]（t=18.124, P=0.000）. Conclusion The high RANKL expression in peripheral blood is probably correlated with aseptic loosening of the implant in patients undergoing THA, which possibly is the prognostic factor of aseptic loosening of the implant.

关 键 词：人工全髋关节置换术 无菌性松动 NF-κB受体活化因子配体 

分 类 号：R687.4[医药卫生—骨科学]