小麦-中间偃麦草抗条锈病渗入系的分子细胞学鉴定  

Cytogenetic and Molecular Identification of a Wheat(Triticum aestivum)-Thinopyrum intermedium Introgression Line with Resistance to Stripe Rust

作　　者：詹海仙[1,2] 畅志坚[2] 李光蓉[1] 贾举庆[3] 郭慧娟[2] 张晓军[2] 李欣[2] 乔麟轶[2] 杨足君[1] 

机构地区：[1]电子科技大学生命科学与技术学院,成都610054 [2]山西省农业科学院作物科学研究所,太原030031 [3]山西农业大学农学院,太谷030801

出　　处：《农业生物技术学报》2014年第7期841-845,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31171839);山西省留学基金项目(No.2012-102);山西省国际合作项目(No.2012081006-2)

摘　　要：小麦品系CH5383是源于中间偃麦草(Thinopyrum intermedium)的兼抗多种小麦病害的新种质。为明确其抗性来源和外源DNA片段的渗入位置,采用基因组原位杂交(GISH)和荧光原位杂交(FISH)对CH5383进行分析,GISH分析未发现外源信号,FISH结果观察到CH5383的3BL染色体端部与对照小麦(Triticum aestivum)品种中国春相比有明显的重组信号,初步推断CH5383染色体3BL端部可能有中间偃麦草DNA片段插入。用135对PLUG(PCR-based landmark unique gene)标记对CH5383、中国春和多个近缘物种进行扩增分析,发现位于3B染色体长臂端部的引物TNAC1383,能在CH5383和中间偃麦草中扩增出大约1 300 bp的特异DNA产物。从而进一步证实,CH5383是一个小麦-中间偃麦草小片段渗入系,携带抗条锈病基因的外源中间偃麦草DNA渗入片段位于3B染色体端部0.81-1.00区段。CH5383可以作为优异的小麦抗病育种新种质加以利用。Wheat（Triticum aestivum）-Thinopyrum intermedium introgression line CH5383 is high resistance to wheat stripe rust and powdery mildew.It was characterized by genomic in situ hybridization （GISH）,fluorescence in situ hybridization （FISH） and molecular marker analysis.GISH using Th.intermedium DNA as probe couldn＇t detect the alien signals.Strong hybridization signal in the distal region of wheat chromosome 3BL was observed when FISH using pHvG38 containing the simple GAA repeat as a probe.A total of 135 PLUG primer pairs were used to test in wheat Chinese Spring （CS）,CH5383 and wheat related species.The results showed that TNAC1383 displayed unique 1 300 bp bands of CH5383 and Th.intermedium.Experimment also verified that CH5383 was a small wheat-alien introgression line and the alien Th.interrnedium DNA segments were possibly located in wheat bin 3BL-0.81～1.00 region.Due to its good diseases resistance,CH5383 can be useful in breeding programs.

关 键 词：小麦 中间偃麦草 渗入系 原位杂交 PLUG标记 

分 类 号：S512.1[农业科学—作物学]