不同种源黑龙骨遗传关系的SRAP分析  

Genetic Relationship of Peripioca forrestii Schltr. from Different Resources by SRAP Analysis

作　　者：刘志飞[1] 高洁[1,2] 滕中华[1] 张正圣[1] 刘德新[1] 坚玲玲 

机构地区：[1]西南大学农学与生物科技学院,重庆400715 [2]南方山地农业教育部工程研究中心,重庆400715

出　　处：《农业生物技术学报》2014年第7期862-869,共8页Journal of Agricultural Biotechnology

基　　金：重庆市科技攻关项目(CSTC;2008AB1001);高等学校学科创新引智计划项目(No.B12006);西南大学人才引进项目(No.207011)

摘　　要：黑龙骨（Periploca forrestii Schltr.）是云贵高原一种传统民族药材,为了确定不同种源的黑龙骨遗传差异,本研究利用相关序列扩增多态性（sequence-related amplified polymorphism,SRAP）分子标记技术,对中国西南地区（云南、贵州、重庆、四川）的11个黑龙骨和4个青蛇藤（Pcalophylla （Wight） Falc.）材料首次进行遗传多样性分析.筛选出40对随机引物组合,对15份材料的基因组DNA进行扩增,共获得420条清晰可辨的扩增条带,其中多态性条带有333条,占79.29％;材料间的遗传距离为0.040～1.613,平均值为0.840,遗传相似系数（genetic similarity coefficient,GS）为0.391～0.973,平均值为0.718,表明供试材料的遗传多样性较丰富.非加权配对类平均法（unweighted pair-group method with arithmetic mean,UPGMA）聚类分析发现,在遗传相似系数为0.59的水平上,可将15份供试材料聚为黑龙骨和青蛇藤2大类,在遗传相似系数为0.80的水平上,可将11个黑龙骨样本聚为3个地理类群：云南滇杠柳、贵州黑骨藤和重庆四川黑龙骨,相同或相似生态地理环境类群的材料基本能聚为一类,呈现出一定的地域性分布特点.本研究结果为黑龙骨种质的收集、利用及育种提供了理论依据.Periplocaforrestii Schltr.is one of the national medicine in the Yunnan-Guizhou plateau of China.In order to assess the genetic diversity of Periploca forrestii Schltr.from different geographic population at molecular levels,sequence-related amplified polymorphism （SRAP） molecular markers were used to detect the genetic diversity of 11 P.forrestii Schltr.accessions and 4 P.calophylla （Wight） Falc.accessions for the first time mainly from Southwest China （Yunnan,Guizhou,Chongqing and Sichuan）.Forty primer pairs had been selected to identify the polymorphism among the 15 test materials and 420 distinguishable DNA fragments were amplified,of which 333 bands were polymorphic.The percentage of polymorphic bands in average was 79.29％.The range of genetic distance of the test accessions was 0.040～1.613 with an average of 0.840,and the genetic similarity coefficient（Gs） of them was 0.391～0.973 with an average of 0.718.All of these suggested that the genetic diversity was comparatively rich among the test materials.The 15 accessions were classified into 2 cluster groups of P.forrestii Schltr.and P.calophylla （Wight） Falc.by cluster analysis using UPGMA（unweighted pair-group method with arithmetic mean） at the similarity coefficient of 0.59.At the similarity coefficient of 0.80,the 11 accessions of P.forrestii Schltr.could be furtherly classified into 3 subgroups which reflected the 3 geographical origins of Yunnan,Guizhou,Chongqing-Sichuan.Moreover,accessions from the same origin frequently clustered into one group,showing a certain degree of ecogeographical distribution.Results of this study will provide references for the collection,breeding and conservation of P.forrestii Schltr..

关 键 词：黑龙骨 SRAP 遗传关系 聚类分析 

分 类 号：S566.301[农业科学—作物学]