模拟失重30 d后再超重对猴牙龈组织趋化因子CCL20及其受体CCR6表达的影响  

Effects of hypergravity exposure after 30 days of simulated weightlessness on chemokine CCL20 and its receptor CCR6 in gingival tissue of rhesus macaque

作　　者：汤楚华[1,2] 牛忠英[2] 郑燕华[3] 包博[2] 洪彪[2] 陈宇晖[2] 

机构地区：[1]解放军医学院,北京100853 [2]解放军第306医院全军口腔疾病诊治中心 [3]特种医学实验中心,北京100101

出　　处：《上海口腔医学》2014年第3期273-279,共7页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30870598);全军医药卫生科研基金(06MA004)~~

摘　　要：目的:探讨模拟失重30 d后再超重对猴牙龈组织趋化因子CCL20及其受体CCR6表达的影响。方法:23只雄性猕猴随机分为4组,即对照组(A)、失重组(B)、超重组(C)、模拟失重30 d后再超重组(D)。其中,D组根据载荷量大小又分为4个亚组,即+11Gx/270 s组(D1)、+13Gx/230 s组(D2)、+15 Gx/200 s组(D3)和+13 Gx/230 s恢复9 d组(D4)。采用组织学方法观察牙龈的形态学变化,通过免疫组织化学、Q-PCR方法检测牙龈组织中CCL20、CCR6的表达情况。采用SPSS17.0软件包对数据进行统计学分析。结果:各实验组中牙龈结构均未见明显损伤,但实验组中的淋巴细胞及中性粒细胞浸润较对照组多。对照组牙龈组织几乎不表达CCL20,但弱表达CCR6;实验组CCL20阳性表达主要位于牙龈上皮组织的基底层,CCR6阳性表达主要位于牙龈上皮棘层和基底层。除失重组的CCL20外,各实验组牙龈组织中的CCL20和CCR6在蛋白水平、mRNA水平上的表达均显著高于对照组(P<0.05)。结论:模拟失重30 d后再超重环境中,猴牙龈组织结构未见明显改变,但牙龈组织趋化因子CCL20及其受体CCR6表达明显增强。PURPOSE： To study the effect of hypergravity exposure after 30 days of simulated weightlessness on the expression of chemokine CCL20 and its receptor CCR6 in gingival tissue of rhesus macaque. METHODS： Twenty-three male rhesus monkeys were randomly divided into 4 groups, namely control group （A, n=3）, weightlessness group （B, n=3）, hypergravity group （C, n=3） and hypergravity exposure after 30 days of simulated weightlessness group （D, n=14）. Group D was further divided into 4 subgroups according to the values of overload as： ＋11 Gx/270 s group （D1, n=3）, ＋13 Gx/230 s group （D2, n=4）, ＋15 Gx /200 s group （D3, n=4） and ＋13 Gx /230 s with 9 days of recovery group （D4, n=3）. Histopathological changes of gingival tissues were observed by hematoxylin-eosin staining and the expressions of CCL20 and CCR6 were detected by immunohistochemistry （IHC） and quantitative real-time PCR （Q-PCR）. SPSS 17.0 software package was used for statistical analysis. RESULTS： Histological observation showed that no significant histopathological change was found in the gingival tissues in all experimental groups. However, there were more infiltrated lymphocytes and nentrophils in the experimental groups. Normal gingival epithelial cells were hardly stained by anti-CCL20 but weakly stained by anti-CCR6. In the experimental groups, CCL20 could be detected in the basal layer of the gingival epithelial tissue, and CCR6 could be detected in the spinous layer and the basal layer of the gingival epithelium. The CCL20 andCCR6 expression in the gingival tissues of each experimental group were significantly higher than those of the control group, not only at the protein level but at the mRNA level （P〈0.05） except the CCL20 expression in the weightlessness group. CONCLUSIONS： Hypergravity exposure after 30 days of simulated weightlessness will not lead to significant pathological changes in gingival tissues, but can induce the expression of ehemokine CCL20 and its receptor CCR6 in gingiva

关 键 词：超重 失重 牙龈 CCL20 CCR6 

分 类 号：R856.78[医药卫生—航空、航天与航海医学]