水稻中两个同源异型结构域转录因子的亚细胞定位  

Subcellular localization of two transcription factors with homeodomain in rice

作　　者：李政龙[1,2] 申奥[1,2] 栾维江[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2014年第3期62-65,共4页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31171515);天津市自然科学基金重点资助项目(11JCZDJC17900)

摘　　要：为了对水稻同源异型结构域转录因子HD-ZipⅢ家族中的HDZ1和HDZ2进行亚细胞定位,利用RT-PCR技术从水稻cDNA中扩增HDZ1和HDZ2的编码区(去除终止密码子序列),与绿色荧光蛋白GFP编码框融合,构建2个转录因子的瞬时表达载体,采用农杆菌介导的转化方法转化至烟草中进行瞬时表达分析.结果表明:PCR扩增所得为目的基因片段HDZ1和HDZ2;二者与载体质粒pCAMBIA35S-GFP连接获得的融合表达载体成功移至烟草中并表达;确定HDZ1主要定位于烟草叶片的气孔中,而HDZ2定位于烟草表皮细胞的细胞膜上.二者在亚细胞中的定位不同,可能在水稻生长发育中所起的作用也不相同.To investigate the subcellular locations of HDZ1 and HDZ2 in flee, which belong to homeodomain transcription factors HD-Zip Ⅲ , the coding regions of HDZ1 and HDZ2 （without the sequence of termination codon） were amplified from eDNA of rice seedlings by RT-PCR, and then fused with green fluorescent protein （GFP） to construct two transient GFP fusion vectors. The analysis of transient expression was carried out by Agrobocterium-mediated transformation into leaves of tobacco. The result showed that： Two genes obtained by PCR were target genes indeed; Both of them were connected with pCAMBIA35S-GFP and transformed into leaves of tobacco by Agrobacterium successfully; It is certain that HDZ1 is mainly localized in stoma of leaves, and HDZ2 localized in cell membrane. HDZ1 and HDZ2 are localized in different subcellular parts, which demonstrate that the two factors may play different roles in development process of rice.

关 键 词：水稻 同源异型结构域HD-ZipⅢ 转录因子 绿色荧光蛋白 亚细胞定位 

分 类 号：Q943.2[生物学—植物学]