钼对镉胁迫下山羊血清抗氧化指标及肝脏凋亡相关基因表达的影响  

Effects of Molybdenum on Serum Anti-Oxidative Indices and Hepatic Apoptosis Gene Expressions of Dairy Goats Challenged by Cadmium

作　　者：庄煜[1] 王琦[1] 曹华斌[1] 胡国良[1] 罗军荣[1] 顾小龙[1] 

机构地区：[1]江西农业大学动物科学技术学院,动物群发性疾病监测和防治研究所,南昌330045

出　　处：《动物营养学报》2014年第7期1822-1829,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金资助项目(31101863);江西省自然科学基金(2010GQN0052);江西省教育厅科学基金资助项目(GJJ10103)

摘　　要：本试验旨在研究钼对镉胁迫下山羊血清抗氧化指标及肝脏凋亡相关基因表达的影响。选用36头健康波尔山羊随机分成4组,每组9头,试验Ⅱ组、试验Ⅲ组和试验Ⅳ组灌服1 mg/kg BW镉,再分别灌服15、30和45 mg/kg BW钼,试验Ⅰ组口服其他3组平均剂量的去离子水。试验期50 d,于第0、10、20、30、40、50天采血测定血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、一氧化氮(NO)含量、一氧化氮合酶(NOS)活性,于第0、25、50天屠宰取肝组织测定B淋巴细胞瘤-2相关X蛋白(Bax)和B淋巴细胞瘤-2(Bcl-2)基因表达量。结果表明:1)与试验Ⅰ组比较,试验Ⅱ组、试验Ⅲ组和试验Ⅳ组均能够显著降低血清T-AOC、SOD活性及肝脏Bcl-2基因表达量(P<0.05),显著升高血清NOS活性、MDA含量、NO含量及肝脏Bax基因表达量(P<0.05);且随着试验天数的增加,血清T-AOC、SOD活性及肝脏Bcl-2基因表达量出现显著下降(P<0.05),血清MDA含量、NO含量、NOS活性及肝脏Bax基因表达量出现显著上升(P<0.05)。2)试验Ⅱ组、试验Ⅲ组和试验Ⅳ组间比较,当镉灌服量一定时,随着钼灌服量的升高,血清T-AOC、SOD活性呈下降趋势,血清MDA含量、NOS活性及肝脏Bax基因表达量呈上升趋势。结果提示,钼、镉可导致山羊血清抗氧化能力的下降同时诱导肝脏凋亡相关基因的表达,钼与镉呈协同关系。This experiment was conducted to determine the effects of molybdenum （Mo） on serum anti-oxidative indices and hepatic apoptosis gene expressions of dairy goats challenged by cadmium （Cd）.Thirty-six healthy Boer goats were randomly divided into 4 groups.Goats in test groups Ⅱ,llⅢ and Ⅳ were all drenched 1 mg/kg BW Cd,and then were drenched 15,30 and 45 mg/kg BW Mo,respectively,while those in test group Ⅰ were drenched demineralized water at the average dose of the other 3 groups.The experimental period was 50 days.Blood collection was carried out on days 0,10,20,30,40 and 50,then serum total antioxidant capacity （T-AOC）,superoxide dismutase （SOD） activity,malondialdehyde （MDA） content,nitric oxide （NO） content and NO synthase （NOS） activity were determined; liver tissue samples were obtained on days 0,25 and 50 after slaughter,then hepatic Bax and Bcl-2 gene expression levels were determined.The results showed as follows：1） compared with test group Ⅰ,serum T-AOC,SOD activity and hepatic Bcl-2 gene expression level could be significantly decreased in test groups Ⅱ,Ⅲ and Ⅳ （P＜0.05）,and serum NOS activity,MDA content,NO content and hepatic Bax gene expression level could be significantly increased in the a bove 3 groups （P＜0.05） ; in addition,with the increase of experimental time,serum T-AOC,SOD activity and hepatic Bcl-2 gene expression level were significantly decreased （P＜0.05）,and serum MDA content,NO content,NOS activity and hepatic Bax gene expression level were significantly increased （P＜0.05）.2） Compared among test groups Ⅱ,Ⅲ and Ⅳ,with the increase of Mo level,serum T-AOC and SOD activity were decreased,and serum MDA content,NO content and NOS activity and hepatic Bax gene expression level were increased when Cd were drenched at the same level.The results indicate that Mo and Cd can decrease serum antioxidant capacity and up-regulate the expressions of hepatic apoptosis genes,and they had a synergetic effect.

关 键 词：山羊 镉 钼 自由基 凋亡基因 

分 类 号：S816[农业科学—饲料科学]