RP-HPLC测定巴洛沙星片的含量  

RP-HPLC Determination of Balofloxacin Content in Its Tablets

作　　者：林生文 

机构地区：[1]广东省食品药品检验所,广州510180

出　　处：《北方药学》2014年第8期11-12,共2页Journal of North Pharmacy

摘　　要：目的：建立RP-HPLC法测定巴洛沙星的含量。方法：采用Ecosil C18色谱柱（250mm×4．6mm，5μm），0．05mol／L磷酸二氢钾溶液（含0．2％三乙胺，用磷酸调节pH值为3．0）：乙腈（78：22），流速为1ml·min^-1，检测波长294nm。结果：采用外标法测定，方法的专属性强，巴洛沙星在48．7-195．0μg·ml^-1范围内呈线性，线性方程为A=52．3268C-5．4290，相关系数为：r=0．9997（n=5）.平均回收率为99．1％（n=9；RSD=0.2％）。结论：该方法准确可靠，重现性好，可用于巴洛沙星片含量的测定。Objective：To establish RP-HPLC method for the determination of Balofloxacin Content in Its Tablets. Method： The test used a Ecosil ClS column. The mobile phase consisted of 0.05mol/L potassium dihydrogen phosphate solution （containing 0.2% three triethylamine, pH value is adjusted to 3 with phosphoric acid） ：acetonitrile （78：22）. The flow rate was 1ml·min^-1, detection wavelength was at 294nm. Result ： Main component self-compare can be used when testing related substances. The method was sensitiveness and specificity. Balofloxacin had a good linear relation in the range of 48.7-195.0μg· ml^-1, the calibration curve was A=52.3268C-5.4290,r= 0.9997 （n=5）,The average recovery rate was 99.1%（n=9; RSD=0.2%）. Conclusion： The method is accurate and reliable,sensitive and rapid and it can be used for the determination of determination of balofloxacin content in its tablets.

关 键 词：HPLC 巴洛沙星片 含量 

分 类 号：R927.2[医药卫生—药学]