HPLC法测定牛蒡子提取物中咖啡酰奎宁酸类化合物的含量  

Content Determination of Caffeoylquinic Acids Compounds from the Extractive of Fructus Arctii by HPLC

作　　者：陈海芹[1] 胥秀英[1] 张心蕊[1] 郑一敏[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400050

出　　处：《北方药学》2014年第8期12-13,共2页Journal of North Pharmacy

摘　　要：目的：以高效液相色谱法测定牛蒡子提取物中咖啡酰奎宁酸类化合物的含量。方法：色谱柱为Welch welchromC18（250mm×4.6mm，5μm），流动相为乙腈：0.1%磷酸（乙腈16%～18%0～60min梯度洗脱乙腈20%65min等度洗脱），流速为1.0ml·min-1，检测波长为327nm，柱温为室温。结果：3，5-二咖啡酰奎宁酸、1，5-二咖啡酰奎宁酸、3，4-二咖啡酰奎宁酸的进样量分别在4.28～12.84μg（r=0.9992）、4.16～12.48μg（r=0.9991）、4.20～12.60μg（r=0.9992）范围内与各自峰面积积分值呈良好线性关系；平均回收率分别为97.70%、98.50%、98.62%，RSD为1.80%、1.90%、1.20%。结论：本方法简便、快速、准确，可用于牛蒡子提取物中咖啡酰奎宁酸类化合物的质量控制。Objective：To determine the contents of Caffeoylquinic Acids Compounds from the extractive of Fructus Arctii by HPLC. Methods：The determination was performed on Welch welchromC18 （250mm ×4.6mm,5μm）column with mobile phase consisted of acetonitrile：0.1% phosphoric acid [acetonitrile16%~18% 0~60 min gradient elution, acetonitrile20% 65 min isocratic elution],at flow rate of 1.0ml·min-1.The detection wavelength was set at 327 nm and the column temperature was room temperature. Results：The linear range of 3,5-dicaffeoylquinic acid was 4.28~12.84μg （r=0.9992）with an average recovery of 97.70%（RSD=1.80%,n=6）. The linear range of 1,5-dicaffeoylquinic acid was 4.16~12.48μg （r=0.9991）with an average recovery of 98.50%（RSD=1.90%,n=6）. The linear range of 3,4-dicaffeoylquinic acid was 4.20~12.60μg（r=0.9992）with an average recovery of 98.62%（RSD=1.20%,n=6）.Conclusion：The method is simple, rapid and accurate for the quality control of Caffeoylquinic Acids Compounds in the extractive of Fructus Arctii.

关 键 词：牛蒡子 咖啡酰奎宁酸 含量测定 

分 类 号：R927.2[医药卫生—药学]