维拉帕米阻抑UUO大鼠肾间质纤维化  

Anti-fibrosis Effect of Varapamil on Rat Kidney with Unilateral Ureteral Obstruction

作　　者：万青松[1] 谢红萍[1] 杨波[1] 

机构地区：[1]南华大学附属第一医院肾内科,湖南衡阳421001

出　　处：《中南医学科学杂志》2014年第5期440-442,共3页Medical Science Journal of Central South China

基　　金：衡阳市科技局课题(2009 KJ39)

摘　　要：目的探讨维拉帕米对单侧输尿管梗阻(UUO)大鼠肾间质纤维化的影响。方法将24只大鼠随机分为对照组(即假手术组)、UUO模型组、维拉帕米治疗组,采用单侧输尿管梗阻模型,术后8天观察梗阻肾组织病理改变,比色法测定肾组织羟脯氨酸(HYP)、丙二醛(MDA)和超氧化物歧化酶(SOD)含量,并应用放射免疫法检测血浆和肾组织内皮素-1(ET-1)的含量。结果与对照组比较,模型组肾组织HYP和MDA含量明显升高,血浆和肾组织内皮素-1含量明显升高,肾组织SOD含量显著下降(P<0.01),维拉帕米治疗组与模型组相比,肾组织HYP和MDA含量明显下降,血浆和肾组织内皮素-1下降,肾组织SOD含量显著升高,差异均有显著性(P<0.05)。结论维拉帕米能减少单侧输尿管梗阻大鼠脂质过氧化物的产生,降低血浆和肾组织内皮素-1的含量,增加抗氧化酶的含量,从而阻抑UUO大鼠肾间质纤维化。Objective To investigate the effect of varapamil on kidney of rats with interstitial fibrosis after unilateal ureteral obstruction（UUO）. Methods Twenty-four rats were randomLy assigned to shame operation group（ normal group）, UUO group and varapamil treatment group after unilateral ureteral obstruction. Renal tissues were examined by light microsco- py 8 days after operation. Hyd -roxyl puamino acid （HYP）, Malondiadehyde （MDA） and superoxide dismutase （SOD） content were measured. Endothelin （ ET-1 ） was measured by radioimmunoassay. Results In comparison with shame operation group, HYP and MDA content significantly increased ,the level of plasmic and renal endothelin-1 significantly increased ,SOD content significantly decreased（ P 〈0.01 ）. In comparison with UUO group, varapamil signicantly ameliorated what had men- tioned （P 〈 0.05）. Conclusions Varapamil can suppress renal interstitial fibrosis by reducing lipid peroxidation production and reducing the level of plasmic and renal endothelin-1 and improving antioxidative enzyme contents.

关 键 词：肾间质纤维化 氧化应激 内皮素-1 维拉帕米 

分 类 号：R692[医药卫生—泌尿科学]