不同浓度脂多糖诱导绵羊免疫器官中NK-Lysin基因表达的研究  

Study on Expression of NK-Lysin Gene in Immune Organs of Sheep Induced by Lipopolysaccharide with Different Concentrations

作　　者：张殿卿[1] 李蕊[1] 陈鹏博[1] 赵庆亮[1] 张国奇[1] 李尤简 盛金良[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《中国畜牧兽医》2014年第7期44-49,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(230960276;31360589)

摘　　要：为研究病原体侵染动物机体时抗菌肽NK-Lysin在机体反应过程中所起到的防御作用及作用机理,本研究通过实时荧光定量PCR检测NK-Lysin在脂多糖(lipopolysaccharide,LPS)不同诱导条件下及不同免疫器官中的相对表达量,探讨绵羊抗菌肽NK-Lysin在免疫器官的生成和应答反应。构建目标基因NK-Lysin和内参基因GAPDH的克隆载体,绘制出标准曲线,检测NK-Lysin的表达量。结果显示,在LPS浓度为0.01、0.1、1、10μg/mL时,NK-Lysin mRNA表达量均在8h内达到最大值,NK-Lysin基因的表达量与LPS的浓度不存在正相关性;NK-Lysin mRNA在肩前淋巴结、脾脏及血液中均有不同程度的表达,脾脏中表达量与其他组织中的表达量相比差异极显著(P<0.01)。免疫器官能在较短时间内表达出抗菌肽NKLysin,使其参与机体先天性免疫应答反应。In order to study the defensive role and action mechanism of antibacterial peptide N K-Lysin during body reacnon,when pathogen infect animal body. This study detected the expression of NK-Lysin induced by different concentrations of li-popolysaccharide （LPS） and in different immune organs by Real-time fluorescence quantitative PCR, and explored the genera-tion and response of sheep antimicrobial peptide NK-Lysin in immune organs. The cloning vector of target gene NK-Lysin andreference gene GAPDH were constructed, drew the standard curve and detected the expression of NK-Lysin. The resultsshowed that inducing with the LPS concentrations of 0.01, 0.1, 1 and 10 μg/mL, respectively,the expression of NK-Lysinreached the maximum within 8 h. There was no positive correlation between the expression of NK-Lysin and concentration ofLPS. The expression of NK-Lysin mRNA had varying degrees in pre-shoulder lymph, spleen and blood. The expression inspleen was extremely significantly different compared with other organs （P〈0.01）. Immune organs could express the antimi-crobial peptide NK-Lysin in a relatively short time, and NK Lysin participated in the body＇s innate immunes responses.

关 键 词：NK-1 ysin 实时荧光定量PCR 脂多糖 免疫器官 表达 

分 类 号：Q786[生物学—分子生物学]