犬弓形虫抗体间接ELISA检测方法的建立  

Establishment of Indirect ELISA Detection Method for Toxoplasma gondii Antibody in Dogs

作　　者：贾艳[1] 陈玉环 杨丽娜 刘跃生[1] 

机构地区：[1]嘉兴职业技术学院,浙江嘉兴314036 [2]北京派克英生物技术有限公司,北京102200

出　　处：《中国畜牧兽医》2014年第7期90-94,共5页China Animal Husbandry & Veterinary Medicine

基　　金：嘉兴市科技局项目(2013AY21035)

摘　　要：试验以利用重组弓形虫SAG1基因转染蜥蜴利什曼原虫所表达获得的目的蛋白作为包被抗原,建立了一种快速、特异、可检测犬弓形虫抗体的间接ELISA方法。确定了抗原最佳包被浓度为6.75μg/mL,血清最佳稀释度为1∶100,对已知阳性血清检测的下限可达1∶6400,批间和批内重复性试验的变异系数均小于10%,包被抗原的酶标板在4、-20℃环境中可保存8个月以上。建立的间接ELISA方法应用于犬弓形虫抗体的检测具有较好的敏感性及特异性。The assay was aimed to establish a rapid and specific indirect ELISA method for canine Toxoplasma gondii anti-body detection,using expressed protein of recombinant Toxoplasma gondii SAG1 gene transfected into Leishmania tarentolaeas the coating antigen. The optimal antigen coating concentration was 6.75 μg/mL, serum optimal dilution was 1 ： 100, theknown positive serum detection was more than 1 - 6400, the coefficients of variation for intra-assay and inter-assay were bothless than 10%, and the coated ELISA plate could be stored more than 8 months at 4 and --20 ℃. The present indirect ELISAdetection method was sensitivity and specificity for detecting Toxoplasma gondii antibody in canine.

关 键 词：犬 弓形虫抗体 间接ELISA 

分 类 号：S852.4[农业科学—基础兽医学]