饲料中牛、羊源性成分现场可视化检测方法的建立  

Development of Visual Loop-mediate Isothermal Amplification Assay for Bovine and Ovine-derived Materials in Feedstuff

作　　者：朱珠 兰青阔 赵新[1,2] 陈锐 郭永泽 王永[1,3] 

机构地区：[1]天津市农业质量标准与检测技术研究所,天津300381 [2]天津大学化工学院,天津300072 [3]河北农业大学植物保护学院,河北保定071001

出　　处：《中国畜牧兽医》2014年第7期95-98,共4页China Animal Husbandry & Veterinary Medicine

基　　金：农业科技成果转化资金项目(2011GB2A100011);科技部国际合作项目(2006DFA32380)

摘　　要：根据牛、羊线粒体中物种特异性序列设计引物,应用可视化环状等温扩增技术(visual loop-mediate isothermal amplification,vLAMP)对牛、羊源性成分进行快速扩增及可视化判断,同时建立牛、羊源成分人工污染的饲料模型,考察vLAMP检测方法的灵敏度。结果表明,牛源性引物和羊源性引物仅分别对牛、羊源性成分产生特异性扩增,灵敏度高达0.001%。本试验所建立的牛、羊源性成分现场可视化筛查方法具有较高的特异性与灵敏度,操作简单、快速,可用于饲料中牛、羊源性成分污染和食品掺伪的现场可视化检测。Two set of primers were designed according to the mitochondrial DNA sequences of the targets, 8 meat powderwere detected by visual loop-mediate isothermal amplification （vLAMP） for method specificity,and meanwhile the mode of arti-ficially contaminated feed was constructed to evaluate the sensitivity of vLAMP assay. The results showed that the bovine andovine-derived materials had specific amplification, but the other animal origin submitted negative reactions. Sensitivity ofvLAMP assay for bovine and ovine-derived materials was 0. 001%,In conclusion, the vLAMP assay developed in the presentstudy was a specific, sensitive, simple and convenient method for the rapid screening of bovine and ovine-derived materials incontaminated feeds.

关 键 词：牛、羊源性成分 环状等温扩增技术 现场可视化检测 

分 类 号：S816[农业科学—饲料科学]