尼龙袋法和改良体外三步法评定瘤胃保护性赖氨酸的瘤胃降解率及小肠消化率  

Determination of Rumen Degradability and Intestinal Digestibility of Ruminally Protected Lysine by Nolon Bag Technique and Modified in vitro Three-step Procedure

作　　者：刘保仓[1] 陈傲东[1] 张博文[1] 孔平[1] 刘晨黎[1] 高巍[1] 

机构地区：[1]石河子大学动物科技学院,新疆石河子832000

出　　处：《中国畜牧兽医》2014年第7期108-111,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家科技支撑计划(2012BAD43B01);兵团博士资金专项(2013BB018)

摘　　要：本试验旨在利用尼龙袋法和改良体外三步法评定脂肪粉包被赖氨酸（Lys）的瘤胃降解率及小肠消化率。选用3只安装有永久性瘤胃瘘管的体重为（28．04±2．97）kg的5月龄哈萨克公羊，代谢笼内单笼饲养，每天饲喂棉籽壳200 g、精料500 g、小麦秸400- 500 g，余料不超过10％。结果表明，该脂肪粉包被赖氨酸的干物质（DM）及粗蛋白质（CP）在瘤胃内的有效降解率均为20％，瘤胃保护率达到80％。经胃蛋白酶和胰酶培养24 h后，DM及CP的消化率分别达到63．6％和98．8％。综上所述，该脂肪粉包被赖氨酸可作为一种新型有效的反刍动物氨基酸补充形式。This trial was conducted to evaluate the rumen degradability and intestinal digestibility of the fat dry powder coa-ted lysine by nylon bag technique and standard three-step in vitro procedure. Three and 5 months old rumen cannulated Kazakhwethers weighed in average （28.0±2.97）kg body weight were housed in metabolic crate singly. The daily diet was composed ofcottonseed hull 200 g, concentrate 500 g, wheat straw 400 to 500 g, and the daily refusals were less than 10%. The resultsshowed that the effective degradability of DM and CP of fat dry powder coated lysine in the rumen were all about 200% ,the ru-men bypass rate achieved 80% or more. After incubation in the pepsin solution and pancreatin solution for 24 h,the in vitro in-testinal digestibility of DM and CP were 63.6% and 98.8% ,respectively. In summary,This fat dry powder coated lysine couldbe used as a novel and effective amino acid supplement in the form of ruminant animals.

关 键 词：瘤胃保护性赖氨酸 有效降解率 小肠消化率 瘤胃 绵羊 

分 类 号：S816[农业科学—饲料科学]